This study was carried out to investigate the effect of alcoholic extract of Lagerstroemia speciosa (L) pers. against carbon tetrachloride (CCl4) induced liver fibrosis in male albino Wistar rats (150-200gm). Liver fibrosis was induced by twice/week administration of CCl4 at a dose of 1ml/kg body weight, mixed with an equal volume of corn oil. The extent of liver fibrosis was assessed by the co...

The extract of K. pinnata was evaluated for its wound healing activity by using excision wound model in rats. On day 11, animals treated with the ethanolic leaf extract exhibited 86.33% reduction in the wound area, compared to petroleum jelly treated control (69.36%) and the mupirocin treated standard (85.49%). The hydroxyproline content of extract treated animals was higher, as compared to con...

Advanced age, independent of concurrent cardiovascular disease, can be associated with increased extracellular matrix (ECM) fibrillar collagen content and abnormal diastolic function. However, the mechanisms causing this left ventricular (LV) remodeling remain incompletely defined. We hypothesized that one determinant of age-dependent remodeling is a change in the extent to which newly synthesi...

Euphorbia hirta (Euphorbiaceae) has reported antimicrobial, antifungal, antiviral anti inflammatory, anti arthritic and antioxidant effect with presence of polyphenolic and flavonoid compounds lead us to evaluate the wound healing activity of enriched flavonoid fraction. Qualitative phytochemical test and quantitative estimation of total flavonoid and polyphenol content was carried out on ethan...

Changes in the structural and functional properties of collagen caused by advanced glycation might be of importance for the etiology of late complications in diabetes. The present study was undertaken to investigate the influence of oral administration of aqueous pod extract (200 mg/kg body weight) of Phaseolus vulgaris, an indigenous plant used in Ayurvedic Medicine in India, on collagen conte...

Swiss male albino mice were treated subcutaneously with the main cannabinoids (CBN, CBD, delta9-THC) at the dosage of 1 mg/kg per day for 30 days, and with the crude resin. At the end of the treatment, after supramaximal stimulation of the sciatic nerve, a significant decrease of both maximal twitch and tetanus tensions was observed in delta9-THC-treated animals; CBD and resin treatment produce...

The protein content of salt-washed cell walls isolated from etiolated stems of Pisum sativum L. approximately doubled during elongation. In the same period the concentration in the wall of hydroxyproline, hydrazine-labile (= presumably glycosylated) serine, valine, tyrosine, lysine, and histidine increased markedly in comparison with other amino acids. After elongation was completed both the am...

We investigated the effect of aging on the extracellular matrix of the lungs by examining age-associated changes in the elastin and collagen content, and the proportion of types I and III collagen, in the whole lungs of BALB/c and SAMR1 male mice between the ages of 3 and 24 months. The elastin content was determined by the hot alkali method. The hydroxyproline content was measured and assessed...

To determine the presence of any significant structural abnormalities in the glomerular basement membrane (GBM) of diabetic individuals, GBM from normal and diabetic human kidneys were isolated and analyzed chemically and structurally. The amino acid composition of the normal GBM revealed the presence of significant amounts of hydroxyproline, hydroxylysine, glycine, and carbohydrate suggesting ...

There has been a great need for a satisfactory method for the determination of hydroxyproline to facilitate the study of the composition of proteins. Lang (1) and Waldschmidt-Leitz and Akabori (2) developed a calorimetric estimation involving oxidation to pyrrole with sodium hypochlorite and color formation with isatin or p-dimethylaminobenzaldehyde. However, oxidation was said to be incomplete...