نتایج جستجو برای: hydroxybutyrate phb

تعداد نتایج: 4992  

Journal: :Applied microbiology 1971
A B Parsons P R Dugan

Zoogloea ramigera 115 synthesized large amounts of matrix polymer from fructose, galactose, glucose, lactose, mannose, soluble starch, and sucrose when these carbohydrates were used as supplements to a chemically defined medium. All of them supported polymer synthesis to the extent that cultures thickened to a gel. Concentration of carbohydrate nutrients in the range 0.5 to 2.0% was not a criti...

Journal: :Indian Journal of Agricultural Sciences 2022

An experiment was conducted at the Indian Agricultural Research Institute, New Delhi (2017-18) to report identification of halophilic poly-ß-hydroxybutyrate (PHB) producing bacteria and optimization process parameters for maximum PHB recovery. The phylogenetic analysis classified isolates into bacterial phylum α, γ-Proteobacteria, Firmicutes. Halomonas sp. KB7 Stenotrophomonas maltophila B11 pr...

Journal: :Biotechnology progress 1999
J J Hahn A C Eschenlauer U B Sleytr D A Somers F Srienc

Bacterial genes responsible for poly(3-hydroxybutyrate) (PHB) biosynthesis were targeted to plant peroxisomes by adding a carboxy-terminal targeting sequence. The enzymes evidently were transported into peroxisomes, retained their catalytic activity, and reacted with peroxisomally available precursors because PHB synthesis in transgenic plant cells was localized to peroxisomes. Up to 2 mg/g fre...

Journal: :Applied and environmental microbiology 2000
W S Ahn S J Park S Y Lee

Fermentation strategies for the production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes were developed. The pH-stat fed-batch cultures of E. coli CGSC 4401 harboring pJC4, a stable plasmid containing the A. latus PHA biosynthesis genes, were carried out with a concentrate...

2016
Riyaz Sayyed

Biodegradable plastics has gained significant commercial interest in recent years as they are having similar properties to that of the synthetic plastic used now a days, they are completely bio-degradable, are produced from natural (microbial) sources and are biocompatible in human system. These properties make PHB as an ideal candidate for various applications in medical field. The present art...

Journal: :Applied and environmental microbiology 2015
Anna Sznajder Daniel Pfeiffer Dieter Jendrossek

Identification of proteins that were present in a polyhydroxybutyrate (PHB) granule fraction isolated from Ralstonia eutropha but absent in the soluble, membrane, and membrane-associated fractions revealed the presence of only 12 polypeptides with PHB-specific locations plus 4 previously known PHB-associated proteins with multiple locations. None of the previously postulated PHB depolymerase is...

Journal: :Applied and environmental microbiology 2000
M Kansiz H Billman-Jacobe D McNaughton

Fourier transform infrared (FTIR) spectroscopy in combination with the partial least squares (PLS) multivariative statistical technique was used for quantitative analysis of the poly(beta-hydroxybutyrate) (PHB) contents of bacterial cells. A total of 237 replicate spectra from 34 samples were obtained together with gas chromatography-determined reference PHB contents. Using the PLS regression, ...

Journal: :Microbial cell factories 2015
Zhenquan Lin Yan Zhang Qianqian Yuan Qiaojie Liu Yifan Li Zhiwen Wang Hongwu Ma Tao Chen Xueming Zhao

BACKGROUND Poly(3-hydroxybutyrate) (PHB), have been considered to be good candidates for completely biodegradable polymers due to their similar mechanical properties to petroleum-derived polymers and complete biodegradability. Escherichia coli has been used to simulate the distribution of metabolic fluxes in recombinant E. coli producing poly(3-hydroxybutyrate) (PHB). Genome-scale metabolic net...

2013
Rongpeng Li Yuji Jiang Xinfeng Wang Jingjing Yang Yuan Gao Xiaoli Zi Xia Zhang Haofeng Gao Nan Hu

A novel facultative psychrotroph (strain CBS-1), which accumulates poly-β-hydroxybutyrate (PHB), was isolated from soil samples taken from Changbai Mountain, China. Phylogenetic analysis based on 16S rRNA sequence data and Biolog analysis identified strain CBS-1 as Pseudomonas mandelii. Transmission electron micrographs revealed abundant electron-transparent intracellular granules. (1)H-nuclear...

Journal: :Macromolecular bioscience 2013
Silvina Ribeiro-Samy Nuno A Silva Vitor M Correlo Joana S Fraga Luísa Pinto Andreia Teixeira-Castro Hugo Leite-Almeida Armando Almeida Jeffrey M Gimble Nuno Sousa António J Salgado Rui L Reis

Spinal cord injury (SCI) leads to devastating neurological deficits. Several tissue engineering (TE)-based approaches have been investigated for repairing this condition. Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) is found to be particularly attractive for TE applications due to its properties, such as biodegradability, biocompatibility, thermoplasticity and piezoelectricity. Hence,...

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