aflatoxin b i (afb) isa well known hepatocarcinogen in several animal species and probably a causative agent in human hepatocellular carcinoma. humans are exposed to afb by ingesting contaminated food. aflatoxin contamination encountered in human foods is usually at low levels which is difficult to measure by chromatographic methods. therefore in the present study we have developed an immunoass...

Crithidia luciliae, a nonpathogenic trypanosomatid, could provide a good alternative source of antigen for serodiagnosis of Chagas' disease. An enzyme-linked immunosorbent assay showed 100% sensitivity and 83% specificity when 91 human serum samples from Chagas' disease patients and 127 human serum samples from people suffering from toxoplasmosis (21 samples), leishmaniasis (32 samples), system...

A competitive enzyme immunoassay (CEIA) was established and compared with other serological techniques for detecting Coxiella burnetii antibody in camels, goats, and sheep. This technique was evaluated because a conjugated anti-camel immunoglobulin was not available to serve as a direct signal for the demonstration of antigen-antibody reaction. A C. burnetii antibody-positive human serum and a ...

purpose: to investigate the penetration of cefixime, cephalexin and ciprofloxacin into the human eye based on microbial inhibition by human aqueous samples after oral administration. method: sixty patients aged 40 to 75 years who were scheduled for cataract surgery were divided into three equal groups. one group received 500 mg oral ciprofloxacin 24, 12 and 2 hours preoperatively; another group...

conclusions we found that purified locally isolated soluble crude antigens of the rh strain of t. gondii from the peritoneal cavity of mice may be one of the most promising antigens for detection of human toxoplasmosis in routine screening. results indigenous elisa was used to examine 100 serum samples containing anti-t. gondii igg, with a sensitivity of 98% (commercial kits: 100%). another 100...

background: human t-lymphotropic virus type i is the etiologic agent of two distinct human diseases, adult t-cell leukemia or lymphoma and a chronic, progressive demyelinating disorder. the aim of this work was to investigate the seroprevalence of htlv-1/2 among high risk patients (thalassemic and hemodialysis) in charmahal-va-baktiari province, center of iran. methods: using elisa, a total of ...

Abstract We detected severe acute respiratory syndrome coronavirus 2 in an otherwise healthy poodle living with 4 family members who had disease. observed antibodies serum samples taken from the dog, indicating seroconversion. Full-length genome sequencing showed that canine and human viruses were identical, suggesting human-to-animal transmission.

Human pythiosis is an emerging, life-threatening infectious disease, caused by the oomycete Pythium insidiosum. Thailand is an area where human pythiosis is endemic and the genetic blood disorder thalassemia is a predisposing factor. Patients with pythiosis present with arterial occlusions of the lower extremities, corneal ulcers, or chronic cutaneous infections. Diagnosis relies on time-consum...