Few sciences have held out such therapeutic promise and correspondingly stirred so much controversy in countries throughout the world as the developing science surrounding human embryonic stem cells. Since the first reported development of several lines of human embryonic stem cells in 1988, many governments around the world have attempted to address the thorny ethical issues raised by human em...

How has the development of human induced pluripotent stem cells (hiPSCs) modified the trajectory of stem cell research? Here, coauthorship networks of stem cell research articles and analysis of cell lines used in stem cell research indicate that hiPSCs are not replacing human embryonic stem cells, but instead, the two cell types are complementary, interdependent research tools. Thus, we conclu...

Human embryonic stem (ES) cells are undifferentiated pluripotent cells derived from the inner cell mass of blastocyst stage embryos. These unique cell lines have the potential to form virtually any cell type in the body and can be propagated in vitro indefinitely in an undifferentiated state. These cells are capable of forming embryoid bodies (EB) that contain cells from all three embryonic lin...

neurotrauma is one of the main medical problems that affect considerable number of population especially young persons in all countries. neurotrauma not only is a serious threat for life but also creates various disabilities and consequently imposes large costs to society. following central nervous system (cns) injury, tissue damage occurs via primary and secondary mechanisms. despite many atte...

Introduction Based on previous researches, dental pulp stem cells (DPSCs) are easily accessible with limited morbidity after collection. Their embryonic origin, from neural crests, explains their multipotency. DPSCs are primarily derived from the pulp tissues of the teeth.Objective: This study was undertaken to isolate, culture, and characterize two different third molar and first premolar hum...

The author presents an overview (completed on September 15, 2001) of three issues involved in the ethics of human embryonic stem cell therapy: the ethical implications of some of the scientific issues involved, the specific ethical issues of the moral standing of the early human embryo and the problem of cooperation, and a consideration of two public policy issues: should the research go forwar...

BACKGROUND Human embryonic stem cells provide access to the earliest stages of human development and may serve as a source of specialized cells for regenerative medicine. Thus, it becomes crucial to develop protocols for the directed differentiation of embryonic stem cells into tissue-restricted precursors. METHODS AND FINDINGS Here, we present culture conditions for the derivation of unlimit...

the aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. blastocyst stage embryos from pregnant nmri mice were obtained and cultured for 1-2 days in dmem medium. the inner cell masses formed...