Drug discovery today requires the focused use of laboratory automation and other resources in combinatorial chemistry and high-throughput screening (HTS). The ultimate value of both combinatorial chemistry and HTS technologies and the lasting impact they will have on the drug discovery process is a chapter that remains to be written. Central to their success and impact is how well they are inte...

Demonstrating a candidate drug's interaction with its target protein in live cells is of pivotal relevance to the successful outcome of the drug discovery process. Although thymidylate synthase (hTS) is an important anticancer target protein, the efficacy of the few anti-hTS drugs currently used in clinical practice is limited by the development of resistance. Hence, there is an intense search ...

Because of methodological breakthroughs and the availability of an increasing amount of whole-genome sequence data, horizontal transfers (HTs) in eukaryotes have received much attention recently. Contrary to similar analyses in prokaryotes, most studies in eukaryotes usually investigate particular sequences corresponding to transposable elements (TEs), neglecting the other components of the gen...

This paper experimentally investigates the relationship between the operating temperature and the power handling capability (PHC) of a coplanar waveguide (CPW) high-temperature superconducting (HTS) dual-band bandpass filter (DBPF) using a YBCO thin film deposited on a MgO substrate. The PHC is evaluated based on the intermodulation distortion of the HTS-DBPF. Experimental results show that the...

In an induction heater for heating of aluminum billets, the main reason for using high-temperature superconducting (HTS) tapes instead of conventional copper conductors is to reduce the large power losses in the induction coil. In this work we present how to calculate the different loss contributions of an induction heater based on HTS tapes. Calculations of these losses are used in the design ...

Andreev reflection is investigated in layered anisotropic normal metal / superconductor (NE) systems in the case of an energy gap in S not negligible respect to the Fermi energy, as probably occurring in high critical temperature superconductors (HTS). We find that in these limits retroreflectivity, which is a fundamental feature of Andreev reflection, is broken modifying sensitively transport ...

In high throughput screening (HTS) campaigns, the quality and cost of commercial reagents suitable for pilot studies often create obstacles upon scale-up to a full screen. We faced such challenges in our efforts to implement an HTS for inhibitors of the phosphopantetheinyl transferase Sfp using an assay that had been validated using commercially available reagents. Here we demonstrate a facile ...

The National Toxicology Program Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM) provides validation support for US Federal agencies and the US Tox21 interagency consortium, an interagency collaboration that is using high throughput screening (HTS) and other advanced approaches to better understand and predict chemical hazards to humans and the environment. T...

Background: High throughput sequencing (HTS) has become one of the primary experimental tools used to extract genomic information from biological samples. Bioinformatics tools are continuously being developed for the analysis of HTS data. Beyond some well-defined core analyses, such as quality control or genomic alignment, the consistent development of custom tools and the representation of seq...