A new fluorometric enzyme immunoassay for 17beta-estradiol (E2) using biotinylated estradiol (BE) as a probe ligand, is described. In this method, E2 is detected indirectly by a solid-phase avidin-biotin binding assay, in which the biotin is immobilized on a microtiter plate (biotin-plate). After the competitive reaction between E2 and BE for the anti-E2 antibody in solution, the free E2 and BE...

Herein we have demonstrated a novel protocol to integrating two immunoassay procedures for performing a sequential dual-protein determination, based on a chemiluminescence (CL) substrate-resolved technology. We evaluated our method for the sequential determination of S100β and neuron-specific enolase (NSE) by using alkaline phosphatase (ALP) and horseradish peroxidase (HRP) as two different lab...

17-a-hydroxy-progesterone is a clinically important steroid hormone which is mainly secreted by zona fasciculata cells of adrenal glands and is routinely measured to screen the patients of congenital adrenal hyperplasia (CAH), a disorder of the steroid biosynthetic pathway observed due to the malfunctioning of enzymes involved in the conversion of cholesterol to cortisol. The classical form of ...

We have developed an electrochemical immunosensor for the detection of ultratrace amounts of aflatoxin M(1) (AFM(1)) in food products. The sensor was based on a competitive immunoassay using horseradish peroxidase (HRP) as a tag. Magnetic nanoparticles coated with antibody (anti-AFM(1)) were used to separate the bound and unbound fractions. The samples containing AFM(1) were incubated with a fi...

This study investigated the effects of replacing dicalcium phosphate (DCP) with Hazara rock phosphate (HRP) on the growth performance of broiler chickens. The purpose was to determine the maximum level of F that could be well tolerated. The HRP (13.16% P and 2.98% F) was incorporated into a standard corn- and soybean meal-based diet by replacing 0, 25, 50, 75, and 100% of DCP based on P. Each t...

A multiple enzyme immunoassay (multi-EIA) was developed to quantify whole-molecule human choriogonadotropin (w-hCG) and free hCG beta-subunits (hCG-beta) simultaneously. A clone of a specific monoclonal antibody was coupled to solid phase; two other clones of different monoclonal antibodies were conjugated to horseradish peroxidase (HRP; EC 1.11.1.7) and alkaline phosphatase (AP; EC 3.1.3.1), r...

One novel electrochemical immunosensor was constructed by immobilizing capture antibody of alpha-fetoprotein (AFP Ab(1)) on a nafion/nanogold-particle modified glassy carbon electrode. With a sandwich immunoassay, one DNA-derived magnetic nanoprobe, simplified as DNA/(ZMPs-HRP-AFP Ab(2))(n), was employed for the detection of AFP. The fabricated procedure of the proposed biosensor was characteri...

A highly sensitive protein detection method based on a novel enzyme-labeled gold nanoparticle (AuNP) probe has been developed. In this method, we firstly prepared the enzyme-labeled AuNP probe by coating AuNP with antibody, single-stranded DNA (ssDNA), and horseradish peroxidase (HRP). Magnetic microparticle (MMP) functionalized with another antibody was used as capture probe. Then, target prot...

We have developed a humanoid robot HRP-1S that can be controlled its whole body motion. In phase one of Humanoid Robotics Project (HRP) of METI of Japan, Honda R&D Co. Ltd. has produced humanoid robots HRP-1 as humanoid research platforms to establish good applications of humanoid robots in phase two of HRP. However HRP-1 is controlled its legs and arms separately, and it is not suitable for so...