A comparison of methods, two based upon 5-hydroxymethylfurfural determination (colorimetric using a semi-automated procedure and HPLC) and ion exchange chromatography (HbA1 using BioRad method) was performed. The influence of variables on 5-hydroxymethylfurfural determination is reported. The dialysis step appears to be essential for an accurate determination of 5-hydroxymethylfurfural released...

objective: with consideration of lethal effects of aflatoxins specially b1 on human health. estimation of aflatoxin-albumin adduct, as an important marker of aflatoxin exposure, seems essential. the aim of this study is optimization of hplc-fluorescence method for measurement of this important marker in blood serum. materials and methods: in this study, blood serum of three groups of rats as a...

an agar well diffusion bioassay method for determination of clarithromycin in human plasma, using micrococcus luteus atcc 9341 as the assay organism, was compared with a selective high-performance liquid chromatographic (hplc) method with uv detection. spiked plasma was used to prepare standard and control samples for both methods. the results of the bioassay analyses with spiked plasma samples...

Two new chemiluminescence methods are described for the determination of herbicide MCPB. Firstly, a flow injection chemiluminescence (FI-CL) method is proposed. In this method, MCPB is photodegraded with a UV lamp and the photoproducts formed provide a great chemiluminescence signal when they react with ferricyanide in basic medium. Secondly, an HPLC chemiluminescence (HPLC-CL) method is propos...

An high-performance liquid chromatography (HPLC) method for identification of quaternary and non-quaternary compounds (parent compounds, intermediates, by-products, and products) within the synthesis of the acetylcholinesterase reactivator HI-6, the most promising antidote of nerve agent poisonings, is described. This HPLC method could be of high interest as a quick purity control for those who...

A new selective high-performance liquid chromatography (HPLC) method with UV detection for the determination of the investigational triazole voriconazole in human plasma by using acetonitrile precipitation followed by reverse-phase HPLC on a C(18) column was compared with a simple agar well diffusion bioassay method with Candida kefyr ATCC 46764 as the assay organism. Pooled plasma was used to ...

Overview Purpose: To examine the use of targeted principal component analysis (PCA) with HPLC-charged aerosol detector data as a means of determining adulteration of olive oils. Method: Oil samples were analyzed as both untreated and hydrolyzed, using a C30 HPLC method and charged aerosol detection. PCA results were determined using peak area percent values of the acylglycerides and free fatty ...

there are various methods for the analysis of potassium sorbate and sodium benzoate in food products, but a rapid and reliable method for identification of these preservatives in doogh (an iranian traditional dairy drink) is a procedure, in which high performance liquid chromatography (hplc) utilized and followed by uv diode array detection of the two preservatives. the aim of this case study w...