In the present study, an effective method of preparative high-performance liquid chromatography (Prep-HPLC) was established to purify two taxanes in Taxus cuspidata. During experimental operation, effects flow rate, injection volume, and column temperature on purity 10-deacetyltaxol (10-DAT) paclitaxel (PTX) were investigated, optimized conditions as follows: rate 10 mL/min, volume 0.5 mL, 30 °...

Increasing speciation demands in clinical chemistry, toxicology and nutrition have made the determination of the total elements in a sample inadequate; the amount of an element and the chemical forms in which it is present need to be known. Inductively coupled plasma mass spectrometry (ICP-MS) was used after high-performance liquid chromatographic (HPLC) separation, as was electrospray ionizati...

A sensitive and rapid method for determination of angiotensin converting enzyme (ACE) inhibitory activity was developed based on a combination of enzymatic reaction followed by high performance liquid chromatography/electrospray-mass spectrometry (HPLC-ESI-MS) determination of its product. The most commonly used substrate hippuryl-histidyl-leucine (HHL) or hippuryl-glycyl-glycine (HGG) hydrolys...

In this study, an immunoproteomic approach was used to identify immunodominant proteins from Mycoplasma mycoides subsp. capri isolates. Membrane proteins, extracted through TX-114 phase partitioning, were separated using mono- and two-dimensional electrophoresis and detected by Western blotting with pooled sera from naturally infected goats. A total of 27 immunoreactive spots, corresponding to ...

BACKGROUND Bioactive activities of adzuki bean have been widely reported, however, the phytochemical information of adzuki bean is incomplete. The aim of this study was to characterize and quantify flavonoids and saponins in adzuki bean. High performance liquid chromatography with diode array detection and electro spray ionization-tandem multi-stage mass spectrometry (HPLC-DAD-ESI-MSn) were app...

Purine nucleotides serve as immediate building blocks for the synthesis of RNAs and DNA, energy carriers co-substrates in activation many metabolites, regulators cellular metabolism, signaling molecules. Knowledge actual tissue-specific concentration these metabolites their rate is essential to studies focused on a metabolic rewiring pathogenesis different diseases, including carcinogenesis. Th...

Nowadays, the transformation activity of food industry results in generation a huge amount daily discarded vegetables wastes. One those undervalued by-products are produced during post-harvesting and processing process artichokes. In present research, potential artichokes’ bracts stalks have been evaluated as natural source phenolic compounds which could be used bioactive ingredients, among oth...

the chemical components in the decoctions of chinese herbal medicines are not always the same as those in the crude herbs because of the insolubility or instability of some compounds. in this work, a high-performance liquid chromatography (hplc) coupled with electrospray ionization (esi) tandem mass spectrometry method was developed to explore dynamic variation patterns of aconitum alkaloids in...

The extraction of red beetroot (Beta vulgaris) peel betalains and phenolics was compared with two extraction methods and solvents. The content of total phenolics in the extracts was determined according to a modification of the Folin-Ciocalteu method and expressed as gallic acid equivalents (GAE). The profiles of extracts were analysed by high-performance liquid chromatography (HPLC). The compo...

the anthocyanins of the fruit skin of iranian forest pear (sange khouj) were separated, identified and quantified using high-performance liquid chromatography/diode array detection (hplc/dad) and hplc/electrospray ionization /mass spectrometry (hplc/esi /ms). the individual anthocyanins were identified by comparing their mass spectral data and retention times with those of standards and publish...