In data presentation of biochemical investigation the amount of a target protein is shown in the y-axis against the x-axis representing time, concentrations of various agents, or other parameters. Western blot is a versatile and convenient tool in such an analysis to quantify and display the amount of proteins. In western blot, so-called housekeeping gene product(s), or "housekeeping proteins,"...

Transcription of genes can be discontinuous, occurring in pulses or bursts. It is not clear how properties of transcriptional pulses vary between different genes. We compared the pulsing of five housekeeping and five developmentally induced genes by direct imaging of single gene transcriptional events in individual living Dictyostelium cells. Each gene displayed its own transcriptional signatur...


 The aim of this study is to determine whether fresh graduates vocational higher education who major in hospitality need opportunities work four-star hotels Bukittinggi, (2) determining the competence workforce for SMK majoring Hospitality be accepted into Bukittinggi City as workers, (3) absorption rate Bukittinggi. research used qualitative approach using Exploration method. This was co...

BACKGROUND AND AIM Sexual differences in the transcript levels of various genes including the hepatic isoforms of cytochrome P450 have been extensively studied. Expression of these sexual dimorphic genes have been quantified by Northern blotting, nuclear run on assays and reverse transcriptase-polymerase chain reaction (RT-PCR) methods using numerous housekeeping genes to normalize results. Ear...

conclusions this study indicates that satureja khuzestaniea essence has inhibitory effects on the gene expression of antibiotic resistance in bla-oxa-23 that has a high mic. given that this essence has a good inhibitory effect on gene expression in mdr acinetobacter baumannii and bla-oxa-23, the results indicate that it could be used as a natural way to prevent the growth of acinetobacter bauma...

Careful normalization is essential for the accurate quantitation of mRNA levels in biopsy-sized tissue samples. Commonly, normalization of the target gene with an endogenous standard, mainly housekeeping genes (HKGs), is applied. However, differences in the expression levels of endogenous reference genes have been reported between different tissues and pathological states. Therefore, we were ch...

Peripheral nerve injury triggers the dysregulation of a large number of genes at multiple sites, including neurons, peripheral nerve stump, and the target organ. Housekeeping genes were frequently used as reference genes to normalize the expression values of target genes. Suitable selection of housekeeping genes that are stably expressed after nerve injury minimizes bias elicited by reference g...

Since paternal age at reproduction is considered to be the main factor determining human spontaneous mutation rate (Crow, J. (1993) Environ. Mol. Mutagenesis, 21, 122-129), the effect of paternal age on human longevity was studied on 8,518 adult persons (at age 30 and above) from European aristocratic families with well-known genealogy. The daughters born to old fathers (50-59 years) lose about...