HCP (hybrid-cluster protein) contains two Fe/S clusters, one of which is a hybrid [4Fe-2S-2O] cluster. Despite intensive study, its physiological function has not been reported. The Escherichia coli hcp gene is located in a two-gene operon with hcr, which encodes an NADH-dependent HCP reductase. E. coli HCP is detected after anaerobic growth with nitrate or nitrite: possible roles for it in hyd...

introduction: legionnair's disease bacterium is a gram negative, facultative intracellular bacilli which parasitize human alveolar macropages as well as some species of free living amobae. this study was achieved in order to compare the cell wall composition in intracellular bacteria. whole cell and outer membrane proteins and the bacterial lipopolysacharide (lps) were analyzec on sds page syst...

Dietary heme iron is an important nutritional source of iron in carnivores and omnivores that is more readily absorbed than non-heme iron derived from vegetables and grain. Most heme is absorbed in the proximal intestine, with absorptive capacity decreasing distally. We utilized a subtractive hybridization approach to isolate a heme transporter from duodenum by taking advantage of the intestina...

Type VI secretion systems (T6SSs) have recently been recognized as potential virulence determinants of many Gram-negative bacterial pathogens. Although mechanistic studies are lacking, T6SS-dependent phenotypes can be observed in various animal models of infection. Presumably translocation of T6SS effectors into target cells is involved in virulence, but few such effectors have been identified....

back ground: candida albicans is a member of the normal human microflora. c. albicans cell wall is composed of several protein and carbohydrate components which have been shown to play a crucial role in c. albicans interaction with the host immune system. major components of c. albican cell wall are carbohydrates such as mannans, β glucans and chitins, and proteins that partially modulate the h...

What’s in your product? Come hear the latest advances in the field of biopharmaceutical HCP Testing ● Rare Reagents: Multiple Case Studies on how to develop your rare reagents, including selection of null cell lines, developing immunization strategies and assessing the specificity of your antibodies, characterization of antibodies and standards by 2-D Gels, western blots, and immunofractionation

What’s in your product? Come hear the latest advances in the field of biopharmaceutical HCP Testing ● Rare Reagents: Multiple Case Studies on how to develop your rare reagents, including selection of null cell lines, developing immunization strategies and assessing the specificity of your antibodies, characterization of antibodies and standards by 2-D Gels, western blots, and immunofractionation

background contamination of therapeutic recombinant proteins with residual host cell dna must be controlled under the regulatory standards. objectives the current study established a new rapid, sensitive real time polymerase chain reaction (pcr) approach to measure the reliably of the residual escherichia coli (e. coli) host cell genomic dna in the recombinant streptokinase and alfa interferon ...

Vibrio fluvialis is an emerging foodborne pathogen of increasing public health concern. The mechanism(s) that contribute to the bacterial survival and disease are still poorly understood. In other bacterial species, type VI secretion systems (T6SSs) are known to contribute to bacterial pathogenicity by exerting toxic effects on host cells or competing bacterial species. In this study, we charac...