The capping of Concanavalin A (Con A) receptors induced by Con A was studied using human peripheral blood lymphocytes. The effects of Con A dose (5--100 micrograms/ml), pretreatment at 4 degrees C, unbound Con A, extracellular Ca2+ and Mg2+ and vinblastine were evaluated using Con A-horseradish peroxidase and electron microscopy. Lymphocytes incubated with Con A at 4 degrees C and fixed with gl...

The quenching by molecular oxygen of the fluorescence from a protoporphyrin IX adduct of horseradish peroxidase has been investigated using both intensity and time-resolved techniques. The bimolecular quenching rate constant determined for this process, as evaluated by the conventional Stern-Volmer analysis, was 2 x 10(8) M-1 s-1, among the lowest observed for protein systems. This result sugge...

Paramagnetic complexes of iron (III), manganese (II), and cobalt (II) protoporphyrins IX and apohemoproteins react with nitric oxide to form spectroscopically distinct compounds. Optical and electron paramagnetic resonance (EPR) studies suggest that a l-electron transfer between nitric oxides and metal ions which occurs in these reactions results in the formation of spin-paired complexes of the...

Seven peroxidase isozymes were isolated from horseradish roots and purified to homogeneity as ascertained by chromatography, ultracentrifugation, and polyacrylamide disk electrophoresis. Purification procedures included ammonium sulfate precipitation and column chromatography on carboxymethyl and diethylaminoethyl cellulose. Peroxidase activity in the seven isozymes accounted for 86% of the act...

one of the most important factors responsible for axonal degeneration following spinal cord trauma is ischemia produced by cord compression. previous studies have revealed that omental transposition upon the injured site of the spinal cord could be beneficial in the induction of partial improvement of neuroelectrical and motor function in laboratory animals. the purpose of this study is to veri...