A generic oligodeoxyribonucleotide microchip was used to determine the sequence specificity of Hoechst 33258 binding to double-stranded DNA. The generic microchip contained 4096 oxctadeoxynucleo-tides in which all possible 4(6)= 4096 hexadeoxy-nucleotide sequences are flanked on both the 3'- and 5'-ends with equimolar mixtures of four bases. The microchip was manufactured by chemical immobiliza...

background: spinal cord injury (sci) occurs most often to young men at the peak of their reproductive health. only 10% of sci men can father children without medical assistance due to potential impairments in ejaculation and sperm quality. objective: the main objective of this experimental study was to evaluate the epididymal necrospermia- sperm death, after chronic sci in rat. materials and me...

Purpose: To investigate the effect of Qibai Pingfei capsules (QBPF) medicated serum on apoptosis rat pulmonary artery smooth muscle cells (PASMC) in hypoxic rats, and to determine relationship between that PI3K/Akt/mitochondrial pathway.Methods: Rat PASMCs were isolated, cultured, optimal time concentration QBPF determined by CCK-8 method. Hypoxic rats treated with QBPF, + LY294002, or SC79. Ap...