T helper 1 cells play a major role in protective immunity against mycobacterial pathogens. Since the antigen (Ag) specificity of CD4(+) human T cells is strongly controlled by HLA class II polymorphism, the immunogenic potential of candidate Ags needs to be defined in the context of HLA polymorphism. We have taken advantage of class II-deficient (Ab0) mice, transgenic for either HLA-DRA/B1*0301...

Zinc transporter 8 autoantibodies (ZnT8A) were analyzed in sera from 1,504 subjects as part of the Type 1 Diabetes Genetics Consortium (T1DGC) Autoantibody Workshop. For these participants with type 1 diabetes (T1D), samples were collected within 3 years of T1D diagnosis. ZnT8A were detected in 862 subjects (57.3%), with the highest frequencies and median titers being associated with the shorte...

Abstract Sensitization to nickel (Ni2+) is the most common T cell mediated type IV hypersensitivity, although mechanisms by which Ni2+ alters antigen presentation are not understood. To study how TCR then recognizes bound MHC molecule, we performed paired single RNA sequencing of PBMCs from two highly sensitized subjects orthopedic implants and characterized three specific CD4+ clones. One rest...

Celiac disease is a common severe intestinal disease resulting from intolerance to dietary wheat gluten and related proteins. The large majority of patients expresses the HLA-DQ2 and/or DQ8 molecules, and gluten-specific HLA-DQ-restricted T cells have been found at the site of the lesion in the gut. The nature of peptides that are recognized by such T cells, however, has been unclear so far. We...

BACKGROUND Little is known about the associations between human leukocyte antigens (HLAs) and food allergies. Our aim was to analyze the associations between the HLA class II polymorphism and food allergy using bioinformatics. METHODS A two-step algorithm was developed which mimics the food allergen processing in the human body. In the first step, the allergen is digested by pepsin, trypsin a...

Amaç: Çölyak hastalığı (ÇH), genetik yatkınlığı olan bireylerde, glutene kalıcı duyarlılık sonucu gelişen, otoimmün, sistemik hastalıktır. Bu çalışmanın amacı, ÇH’nin klinik, laboratuar özellikleri ve HLA doku tiplerinin geriye dönük değerlendirilmesidir.Ayrıca lieratür taramasıyla tipleri açısından Türkiye’de bölgelere göre farklılık benzerliklerin incelenmesidir. Yöntem: Çalışmaya, Temmuz 201...

Evaluating risk of developing type 1 diabetes (T1D) depends on determining an individual's HLA type, especially of the HLA DRB1 and DQB1 alleles. Individuals positive for HLA-DRB1*03 (DR3) or HLA-DRB1*04 (DR4) with DQB1*03:02 (DQ8) have the highest risk of developing T1D. Currently, HLA typing methods are relatively expensive and time consuming. We sought to determine the minimum number of sing...

preDQ is a software tool for peptide binding prediction to HLA-DQ2 and HLA-DQ8 proteins specially designed developed the European Food Safety Authority. The able identify peptides and/or predict their affinities. used assess risk of novel cause celiac disease. Predictions made by are based on five models assessment reported outputs. using datasets known non-binding proteins. compiled from liter...

CD4⁺ T cells that recognize dietary gluten antigens presented by the disease-associated HLA-DQ2 or DQ8 molecules are central players in coeliac disease. Unbiased sequencing of the human TCRα variable (TRAV) and humanTCRβ variable (TRBV) genes of 68 HLA-DQ2.5-glia-α2-specific T cells from coeliac disease patients confirmed previous reports of over-usage of the TRBV7-2 gene segment, a conserved A...