We evaluate the ability of hexahistidine (His6) tags on peptide and protein substrates to recruit deoxyribozymes for modifying those substrates. For two different deoxyribozymes, one that creates tyrosine-RNA nucleopeptides and another that phosphorylates tyrosine side chains, we find substantial improvements in yield, kobs, and Km for peptide substrates due to recruiting by His6/Cu(2+). Howeve...

enter the Army, and shortly after finishing his course at Netley was sent in command of a bearer company to the Cape during the Boer war. His next active duty was in the Egyptian campaign of 1882, where lie was surgeon to the 2nd Bearer Company, and was present at the battles of Kassassin and Tel-el-Kebir, receiving the medal and clasp and Khedive's star. He was a typical specimen of the soldie...

This paper proposes an anticipation model of potential customers’ purchasing behavior. This model is inferred from past purchasing behavior of loyal customers and the web server log files of loyal and potential customers by means of clustering analysis and association rules analysis. Clustering analysis collects key characteristics of loyal customers’ personal information; these are used to loc...

http://www.webscience.org/wpcontent/uploads/sites/117/2016/12/ WebSci-manifesto-v19.pdf This paper has been written by Martin Stewart-Weeks, based on his “reflect and report” role at Brave Conversations, a 2-day Web Science 2017 conference held in Canberra, Australia. The conference, convened by Intersticia and the Intersticia Foundation, was supported by the Web Science Trust, the Ethics Centr...

Richard Wright’s introductory essay to his collection of short stories Uncle Tom's Children describes hidden forms of resistance against Jim Crowism and Uncle Tomism. The essay entitled The Ethics of Living Jim Crow: An Autobiographical Sketch relates Wright’s daily confrontations with Jim Crowism with a particular emphasis on his evasive actions, and his covert activities, such as silence, pla...

We present a molecular theory to study the adsorption of different species within pH-sensitive hydrogel nanofilms. The theoretical framework allows for a molecular-level description of all the components of the system, and it explicitly accounts for the acid-base equilibrium. We concentrate on the adsorption of hexahistidine, one of the most widely used tags in bio-related systems, particularly...

This paper considers the following induction problem. Given the background knowledge B and an observation O, find a hypothesis H such that a consistent theory B ∧ H has a minimal model satisfying O. We call this type of induction brave induction. Brave induction is different from explanatory induction in ILP, which requires that O is satisfied in every model of B ∧ H. Brave induction is useful ...