background: the purpose of this research was to determine the relation between negative or positive qualitative troponin i test and the short term prognosis of patients presenting to emergency department with chest pain or angina equivalent symptoms. methods: we assessed the qualitative rapid troponin i in patients with chest pain or angina equivalent symptoms after at least 4 hours and then we...

Methods for preparing troponin and tropomyosin from ethanol-extracted muscle are presented. Examination of these proteins by disc electrophoresis indicated that the troponin preparation is homogeneous. If precautions were taken to assure that the -SH groups of tropomyosin were not permitted to oxidize during preparation or electrophoresis, tropomyosin appeared to be nearly homogeneous. Traces o...

Skeletal-muscle troponin I and troponin T were found to be rapidly phosphorylated by cardiac phospholipid-sensitive Ca2+-dependent protein kinase, with Km values of 6.66 and 0.13 microM respectively. Stoichiometric phosphorylation of skeletal troponin I (endogenous phosphate content 0.7 mol/mol) indicated that the Ca2+-dependent enzyme and cyclic AMP-dependent protein kinase incorporated 0.9 an...

The Troponin Era The advent of the “troponin era” has its roots in the redefi nition of acute myocardial infarction (AMI) by the joint committee of European and American cardiologists in 2000.1 Troponin was identifi ed as the preferred serologic biomarker for acute coronary syndrome (ACS). Since then, troponin assays have continued to evolve with improved sensitivity and reproducibility, leadin...

Cardiospecific troponins are specifically localized in the troponin-tropomyosin complex and cytoplasm of cardiac myocytes. troponin molecules released from myocytes upon their death (irreversible damage acute coronary syndrome) or reversible to myocytes, for example, during physical exertion influence stress factors. Modern high-sensitive immunochemical methods detecting cardiospecific T I extr...

Acute myocardial infarction (AMI) was defined based on clinical findings, ECG abnormalities and cardiac enzymes. However with sensitive marker such as troponin the definition has been refined. In this study two cut-off values of troponin T (0.03ng/mL and 0.1ng/mL) were compared with the final diagnosis of AMI made by the physician. This was a retrospective study to determine the efficiency of c...

BACKGROUND In hemodialysis patients, elevated plasma troponin concentrations are a common finding that has even increased with the advent of newly developed sensitive assays. However, the interpretation and relevance of this is still under debate. METHODS In this cross-sectional study, we analyzed plasma concentrations of sensitive troponin I (TnI) and troponin T (TnT) in stable ambulatory he...

BACKGROUND New-generation high-sensitivity assays for cardiac troponin have lower detection limits and less imprecision than earlier assays. Reference 99th-percentile cutoff values for these new assays are also lower, leading to higher frequencies of positive test results. When cardiac troponin concentrations are minimally increased, serial testing allows discrimination of myocardial infarction...