A self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage ?pL promoter, was constructed. The designed 5024 base pair expression plasmid contained a heat sensitive repressor cI857 coding gene to regulate the function of ?pL promoter under heat shock induction. Using the constructed vector,...

A panel of monoclonal antibodies was raised against the low-affinity human granulocyte-macrophage colony-stimulating factor (hGM-CSF) receptor a-chain expressed as recombinant protein on murine FDC-P1 cells. All the selected antibodies were of the lgG2A isotype and bound to protein A. They each recognized both native and recombinant receptors by indirect surface immunofluorescence and by immuno...

The human granulocyte-macrophage colony stimulation factor (hGM-CSF) gene was cloned in the pET 23a( +) expression vector under the control of strong bacteriophage T7 transcription and translation signals. The hGM-CSF gene was transferred into E. coli strainBL21 (DE3)pLysS andIPTG was used for induction of GM-CSF gene. Production of the target protein was obtained as revealed by ELISA and ...

In order to study the effect of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) as active cytokine through orally administration, we expressed hGM-CSF within silkworm pupae bioreactor. The purified rhGM-CSF named as BmrhGM-CSF is characterized as 29kDa glycoprotein, and its biological activity was measured both in vitro and in vivo. We found out BmrhGM-CSF could stimulate the c...

In this study, Xiushui 134Bt, a highly insect-resistant rice transgenic material with stable expression of CryIAc1 gene in plants and no significant changes main agronomic traits, was obtained by means Agrobacterium-mediated transformation method using japonica 134 as receptor material. Biosecurity the 134Bt assessed rat feeding experiments. Wistar rats were fed nutritionally balanced diet supp...

Computational prediction of in-hospital mortality in the setting an intensive care unit can help clinical practitioners to guide and make early decisions for interventions. As data are complex varied their structure components, continued innovation modelling strategies is required identify architectures that best model outcomes. In this work, we trained a Heterogeneous Graph Model (HGM) on elec...

Waggener GT, Kwon I, Wiley L, Lee Y, Nichols IH, Haymes, E. The Effect of a Low Glycemic vs. High Glycemic Pre-Exercise Meal in Recreationally Trained Endurance Cyclists. JEPonline 2016;19 (2):91-98. The purpose of this study was to examine the effects of two iso-kilocaloric (837.2 kJ) pre-exercise meals: a low glycemic meal (LGM) vs. a high glycemic meal (HGM) and a placebo meal in recreationa...

The cytokine Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) regulates proliferation, differentiation, and apoptosis during myelopoiesis and erythropoiesis. Structure-function relationships of GM-CSF interactions with its receptor (GM-R), the biochemistry of GM-R signal transduction, and GM-CSF action in vivo are relatively well understood. Much less is known, however, about GM-R func...

Pearl millet (Pennisetum glaucum) has potential as a grain crop for dryland crop production in the southeastern United States. Whether or not pearl millet will be compatible in rotation with cotton (Gossypium hirsutum), corn (Zea mays), and peanut (Arachis hypogaea) will depend, in part, on its host status for important plant-parasitic nematodes of these crops. The pearl millet hybrid 'TifGrain...