Both polyethylene glycol (PEG) and mannitol inhibit gibberellic acid-induced alpha-amylase production in barley aleurone layers. The effect of the osmotic solution is on enzyme synthesis rather than alpha-amylase secretion. The inhibition of alpha-amylase synthesis does not appear to be mediated via an indirect effect on respiration or protein synthesis. Rather it seems that the osmotic solutio...

Cordycepin, an inhibitor of RNA synthesis in barley (Hordeum vulgare L.) aleurone cells, does not inhibit the gibberellic acid-enhanced alpha-amylase (EC 3.2.1.1.) synthesis in barley aleurone layers if it is added 12 hours or more after the addition of the hormone. However, the accumulation of alpha-amylase activity after 12 hours of gibberellic acid can be decreased by abscisic acid. The accu...

Amylase is an enzyme that catalyzes the breakdown of starch into sugars and plays a pivotal role in a variety of areas like use as digestives, for the production of ethanol and high fructose corn syrup, detergents, desiring of textiles, modified starches, hydrolysis of oil-field drilling fluids, and paper recycling. In the present work, solid state fermentation (SSF) for α -amylase production h...

The aim of this work was to evaluate the inhibitory activities of methanolic extracts of Artocarpus altilis, Cinnamomum zeylanicum, Piper betel and Artocarpus heterophyllus on Wheat alpha amylase and Baker’s yeast alpha glucosidase at varying concentrations. Diabetes mellitus is a clinical condition characterized by hyperglycaemia in which an elevated amount of glucose circulates in the blood p...

Introduction: α-amylase is a major form of amylase found in humans and other mammals. It is the special key enzyme involved in carbohydrates breakdown. Inhibition of this enzyme could be used in treatment of diabetes. In this study, the effect of ten Iranian macrolichens on alpha amylase were tested. Methods: Different concentrations of the extracts (25, 50 and 75 mg/ml) were incubated wi...

We tested the hypothesis that overweight/obese men aged 50-70 years will have a greater salivary cortisol, salivary alpha amylase and heart rate (HR) responses to psychological stress compared with age matched lean men. Lean (BMI=20-25 kg/m(2); n=19) and overweight/obese (BMI=27-35 kg/m(2); n=17) men (50-70 years) were subjected to a well-characterised psychological stress (Trier Social Stress ...

The current study sought to examine whether children's spontaneous use of the emotion regulation strategies suppression and reappraisal during a psychosocial stress task was related to their cortisol and alpha-amylase responses to that task. Salivary cortisol and alpha-amylase responses to a psychosocial stress task were assessed in 158 10-year-old children (83 girls). The children completed a ...

The gene encoding the hyperthermophilic extracellular alpha-amylase from Pyrococcus furiosus was cloned by activity screening in Escherichia coli. The gene encoded a single 460-residue polypeptide chain. The polypeptide contained a 26-residue signal peptide, indicating that this Pyrococcus alpha-amylase was an extracellular enzyme. Unlike the P. furiosus intracellular alpha-amylase, this extrac...

Highlight: The level of pathogen alpha-amylase and plant beta-amylase activities could be components of plant-pathogen interaction associated with the resistance of triticale to Fusarium head blight. Triticale was used here as a model to recognize new components of molecular mechanism of resistance to Fusarium head blight (FHB) in cereals. Fusarium-damaged kernels (FDK) of two lines distinct in...

Obesity is a main reason of type 2 diabetes and also chronic exposure to arsenic (As)can produce diabetic symptoms. In previous studies, the association between high-fat dietand arsenic in the incidence of diabetes was found, but the role of beta cells activity, livermitochondrial oxidative stress, and hepatic enzymes (leptin, adiponectin and beta amylase)was unclear. Thus, present study was co...