Previously, we have reported that ascorbic acid regulates calcium signaling in human larynx carcinoma HEp-2 cells. To evaluate the precise mechanism of Ca(2+) release by ascorbic acid, the effects of specific inhibitors of the electron transport chain components on mitochondrial reactive oxygen species (ROS) production and Ca(2+) mobilization in HEp-2 cells were investigated. It was revealed th...

In a typical cell culture system, growth factors immobilized on the cell culture surfaces can serve as a reservoir of bio-signaling molecules, without the need to supplement them additionally into the culture medium. In this paper, we report on the fabrication of albumin/heparin (Alb/Hep) assemblies for controlled binding of basic fibroblast growth factor (FGF-2). The surfaces were constructed ...

HEp-2 cells underwent apoptosis when the cells were incubated in medium containing sorbitol. Infection with herpes simplex virus type 1 (HSV-1) prior to the sorbitol treatment suppressed this apoptosis completely, indicating that HSV-1 carries an antiapoptosis gene. In addition, HSV-1 multiplication was restricted in these apoptotic HEp-2 cells.

It has been shown that over-expression of B-cell-specific Moloney murine leukemia virus insertion site 1 (Bmi1) play a key role in maintaining the stem-like properties in CD133+ Hep-2 cells. This study was aimed at investigating the effects of silencing Bmi11 on CD133+ Hep-2 cells. Bmi1 silencing was achieved by transfection with the vectors expressing small hairpin RNA (shRNA) versus Bmi1. Cel...

Chlamydia pneumoniae has proved to be difficult to isolate and propagate in cell culture. We compared the growth of three strains of C. pneumoniae, TW-183 and two clinical isolates from Brooklyn, N.Y., in five cell lines, including HeLa 229, McCoy, HL, HEp-2, and HTED, an immortalized human tracheal cell line. HEp-2 was the most sensitive cell line tested. When 10-fold dilutions of three C. pne...

Progress in the theoretical description of B-meson decays, in particular decays to light hadrons, is reviewed. The factorization properties of such decays can be analyzed using the softcollinear effective theory. Applications of the effective theory to both inclusive and exclusive decays are discussed. FERMILAB-CONF-04-309-T hep-ph/0411065

The present study aimed to identify genes related to 5AZA-CdR in laryngeal squamous cell carcinoma (LSCC) and to investigate the role of S100A4 in the development and aggression of LSCC. Differentially expressed proteins were identified in Hep-2 cells treated with 5AZA-CdR by two-dimensional gel electrophoresis combined with MALDI-TOF-MS. mRNA, protein levels and DNA methylation status of S100A...