The optimal incubation conditions for isolation of group A streptococci from throat cultures are controversial. Therefore, we compared the effects of aerobic and anaerobic incubations after 24 and 48 h on the recovery of group A streptococci. Throat swabs submitted to the clinical laboratory were inoculated onto duplicate 5% sheep blood agar plates, incubated aerobically or anaerobically (GasPa...

During some studies which have been in progress for several years upon the etiology and the clinical course of acute glomerular nephritis, it was considered desirable to examine the serum from these patients for antibodies to hemolytic streptococci. Comment has frequently been made to the effect that infections by this organism involving the upper respiratory tract usually precede the onset of ...

1. Confirming the observations of other experimenters, it has been found that group A hemolytic streptococci produce a capsule containing a polysaccharide which is similar to, if not identical with, certain high molecular weight sugars found in the mammalian body. 2. Leech extract possesses a powerful enzyme capable of splitting one of the linkages in this polysaccharide and of decapsulating gr...

1. A relatively avirulent strain of hemolytic streptococci injected into the skin of normal rabbits seldom diminished and usually increased in number during the first 5 hours after inoculation but after 12 hours save in exceptional instances the organisms diminished rapidly and disappeared. 2. Hemolytic streptococci injected into the skin of normal rabbits passed rapidly from the site of inject...

HOOD, M., JANNEY, A. & DAMERON, G. (1961) Beta-hemolytic streptococcus Group B associated with problems of the perinatal period. American Journal of Obstetrics and Gynecology, 82, 809. JONES, H. EVERLEY & HOWELLS, C.H.L. (1968) Neonatal meningitis due to Streptococcus agalactiae. Postgraduate Medical Journal, 44, 549. KEITEL, H.G., HANANIAN, J., TING, R., PRINCE, L.N. & RANDALL, E. (1962) Menin...

Group B beta-hemolytic streptococci were isolated from the throats of 49 of 1,110 patients who had pharyngitis. Compared with patients whose throat cultures were negative for beta-hemolytic streptococci, those harboring group B were more likely to have enlarged tonsils (P less than 0.001), exudate (P less than 0.02), and tender enlarged anterior cervical lymph nodes (P less than 0.01). Group B ...

When washed streptococci were incubated in a phosphate buffer containing MgSO4 and maltose (Bernheimer's basal medium) and centrifuged, no hemolytic activity was detected in the supernatant. Although incubation of the spent medium with carrier oligonucleotide did not yield active hemolysin, the mixture turned to be significantly hemolytic, upon ethanol precipitation and dehydration. Oxygen stab...

1. No connection can be demonstrated between grouping of non-hemolytic streptococci based upon fermentation reactions, and grouping based upon immunological reactions. 2. A classification of non-hemolytic streptococci can be effected by studying the complement fixation reactions between the streptococci and their antisera. 3. The arrangement of the streptococci in such a classification depends ...