recombinant human interferon alpha 2a (rhifn α -2a) production and cell growth were monitored in a set of genetically modified e. coli strains (msd1519, msd1520, msd 1521, msd 1522, msd 1523) producing rhifn α -2a. the growth was followed at od 600 nm, changes in cell physiology were detected by pyrolysis mass spectrometry (pyms) of cell biomass and recombinant protein production was determined...

CaCl2 treatment followed by heat shock is the most common method for artificial transformation. Here, the cells were transformed using CaCl2 treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. The BL21 strain of Escherichia coli (E. coli) was being susceptible using CaCl2 treatment. Some Cells were ...

Previously we reported that expression of GSH1 (gamma-glutamylcysteine synthetase) and GSH2 (glutathione synthetase) of the yeast Saccharomyces cerevisiae was increased by heat-shock stress in a Yap1p-dependent fashion and consequently intracellular glutathione content was increased [Sugiyama, Izawa and Inoue (2000) J. Biol. Chem. 275, 15535-15540]. In the present study, we discuss the physiolo...

For the military and civilian first responders heat injury is a very real concern. Several real-time physiological monitoring systems exist that can utilize heat strain indexes [e.g. physiological strain index (PSI)] and provide alerts to medical personnel. However, these systems depend on core temperature measurement using ingested pill thermometers which can suffer inaccuracies from ingested ...

BACKGROUND Heat exposure among construction workers in the Persian Gulf region is a serious hazard for health. The aim of this study was to evaluate the performance of wet bulb globe temperature (WBGT) Index for estimation of heat strain in hot/humid conditions by the use of Physiological Strain Index (PSI) as the gold standard. MATERIAL AND METHODS This cross-sectional study was carried out ...

[1] We quantify the energy dissipated to heat and to local disorder in a sheared layer of granular fault gouge. Local disorder is modeled using shear transformation zone theory, a continuum model of nonaffine deformation in amorphous solids that resolves spontaneous localization of strain. Strain localization decreases the total energy dissipated during slip. In addition, a fraction of this ene...

OBJECTIVES The high values of thermal resistance (Rct) and/or vapor resistance (Ret) of chemical protective clothing (CPC) induce a considerable thermal stress. The present study compared the physiological strain induced by CPCs and evaluates the relative importance of the fabrics' Rct, Ret, and air permeability in determining heat strain. METHODS Twelve young (20-30 years) healthy, heat-accl...