We hypothesized that the function of duodenocyte apical membrane acid-base transporters are essential for H(+) absorption from the lumen. We thus examined the effect of inhibition of Na(+)/H(+) exchanger-3 (NHE3), cystic fibrosis transmembrane regulator (CFTR), or apical anion exchangers on transmucosal CO(2) diffusion and HCO(3)(-) secretion in rat duodenum. Duodena were perfused with a pH 6.4...

This work examines the influence of Cl− (2.5 125 mM) and 3 HCO − (2 30 mM) on the Cl−/ 3 HCO − ATPase complex of the neuronal membrane and this enzyme is a Cl−-pump that is coupled to GABAA receptors. The greatest (44%) activating effect on the enzyme is found with 3 HCO − (20 30 mM), while the maximum activity occurs in the presence of a ratio of ~25 mM 3 HCO − /~5mM Cl−. Blockers of the GABAA...

We report the detection of HCO(J=1→0) emission towards the Cloverleaf quasar (z = 2.56) through observations with the Very Large Array. This is the first detection of ionized molecular gas emission at high redshift (z > 2). HCO emission is a star formation indicator similar to HCN, tracing dense molecular hydrogen gas (n(H2) ≃ 10 5 cm) within star–forming molecular clouds. We derive a lensing–c...

Cyclic AMP produced from membrane receptor complex bound adenylyl cyclases is protective in corneal endothelial cells (CEC). CEC also express soluble adenylyl cyclase (sAC), which is localized throughout the cytoplasm. When activated by HCO(3)(-), cAMP concentration ([cAMP]) increases by ∼50%. Here we ask if cAMP produced from sAC is also protective. We examined the effects of HCO(3)(-), pH, ph...

The cyanobacterium Synechocystis sp. strain PCC 6803 possesses two CO(2) uptake systems and two HCO(3)(-) transporters. We transformed a mutant impaired in CO(2) uptake and in cmpA-D encoding a HCO(3)(-)transporter with a transposon inactivation library, and we recovered mutants unable to take up HCO(3)(-) and grow in low CO(2) at pH 9.0. They are all tagged within slr1512 (designated sbtA). We...

While there is some evidence that changes in nicotinic acetylcholine receptor (nAChR) subunits confer resistance to levamisole in gastrointestinal helminth parasites, the exact nature of the resistance mechanism(s) is unclear. We utilised the presence of a resistant fraction within the Wallangra 2003 isolate of Haemonchus contortus larvae in order to subdivide the population into three subpopul...

Naturally occurring tufts of the mixotroph Thiothrix nivea blanketed the East Everglades (Dade County, Fla.) Chekika artesian well and runoff areas. The rate of HCO(3) fixation by these Thiothrix tufts was determined to be 14.0 +/- 5.4 nmol of HCO(3) per min per mg of dry weight, which reflected a growth rate of 5.0%/h. The addition of 10 mM glucose, ribose, acetate, or pyruvate or 0.05% Casami...

Membrane-bound carbonic anhydrase (CA) is critical to renal acidification. The role of CA activity on the basolateral membrane of the proximal tubule has not been defined clearly. To investigate this issue in microperfused rabbit proximal straight tubules in vitro, we measured fluid and HCO(3)(-) absorption and cell pH before and after the extracellular CA inhibitor p-fluorobenzyl-aminobenzolam...

Hepatoid carcinoma of the ovary (HCO) was first reported in 1987 in 5 cases of malignant ovarian tumors which were similar to hepatocarcinoma in the histological analysis. We report the first case in the literature of a patient diagnosed with HCO treated using HIPEC after extensive cytoreductive surgery, and we discuss the value of this therapeutical option in patients with HCO.

HYPOTHESIS Serum bicarbonate (HCO(3)) measurement may accurately and reliably be substituted for the arterial base deficit (BD) assay in the surgical intensive care unit (ICU). DESIGN Retrospective criterion standard analysis. SETTING Surgical ICU in a tertiary care facility. PATIENTS Consecutive sample of non-trauma-related surgical ICU admissions from January 1996 to January 2004 with s...