Plackett-Burman experimental design was used to screen important medium components influencing the production of polyribosyl ribitol phosphate by Haemophilus influenzae type-B under submerged fermentation. Eleven media parameters such as Glutamic acid, Yeast extract, cystein, dextrose, NAD, heamin, NaCl, NH4Cl, Na2HPO4, KCl, and MgSO4.7H2O were screened in twelve experimental runs as per the de...

The widespread use of Haemophilus influenzae type b (Hib) conjugate vaccines has nearly eradicated invasive Hib disease where the vaccines are used. This success was accompanied by a shift in capsular serotypes of invasive H. influenzae disease, with nontypeable strains replacing type b strains as the most common bloodstream isolate, but there is no convincing evidence of a true increase in the...

Utilization of heme-hemopexin as a source of heme by Haemophilus influenzae type b is dependent on expression by this bacterium of the 100-kDa HxuA protein, which is both present on the bacterial cell surface and released into the culture supernatant (L. D. Cope, R. Yogev, U. Muller-Eberhard, and E. J. Hansen, J. Bacteriol. 177:2644-2653, 1995). Radioimmunoprecipitation analysis showed that the...

Background: Streptococcus pneumoniae and Haemophilus influenzae type b (Hib) is a leading cause of bacterial pneumonia, meningitis, and sepsis in children under 5-year of age. This study aims to estimate potentially preventable number of cases and deaths caused by Hib and pneumococcal in Iran.Materials and Methods:We used the burden of diseases model estimates of the World Health Organization (...

Haemophilus influenzae type b (Hib) is the leading cause of meningitis in children < 5 years of age. The majority of cases of Hib occur in infants < 2 years of age. Until recently the only vaccine available against this disease contained the pure polysaccharide (PRP) of Hib (Hib-PRP vaccine). The Hib-PRP vaccine was demonstrated to be efficacious in infants > 18 months of age but not below that...

In summary, all of the Hib conjugate vaccines are highly immunogenic and efficacious in children older than 12-15 months of age, and HbOC, PRP-OMPC, and PRP-T are highly immunogenic and demonstrated to be efficacious in infants as young as 2 months old. HbOC, PRP-OMPC, and PRP-T have been licensed in numerous countries for infants and are recommended for infant immunization. However, perhaps th...

31 cases of invasive Haemophilus influenzae type b infections occurred in adults in Denmark during a period of 2 years and 5 months corresponding to an incidence of 0.3/100,000/year. Only 6 patients had no underlying condition. The incidence of H. influenzae type b infections in adults will probably rise in the future, because the increasing use of therapeutic measures affecting the immune syst...

Since the introduction of type b Haemophilus influenzae vaccination, noncapsulated H. influenzae has become responsible for most cases of invasive H. influenzae diseases. In our two cases of septic arthritis, we isolated strains with β-lactamase-positive amoxicillin-clavulanate resistance (BLPACR). Thus, the increasing prevalence of BLPACR should be taken into account when empirical therapy is ...