The specificity and sensitivity of two commercial enzyme linked immunosorbent assays (ELISAs), Diamedix (Miami, Florida) and Lipogen (Noxville, Tennessee), were assessed and compared with haemagglutination and immunodiffusion assays. Sera from 53 patients with various connective tissue disorders were examined for the presence of antibodies to nuclear antigens (ANA), double stranded DNA (dsDNA),...

The present study was designed to investigate effects of Lisovit (Biomin, Austria) on the immune system of broiler chickens. For this purpose, 270 (day-old) broiler chicks were randomly divided into three groups A, B and C of 90 birds each. The birds were vaccinated against Newcastle Disease (ND) on day 3 (intraocular) and day 18 (drinking water) of age and against Infectious Bursal Disease (IB...

this experiment was conducted for evaluating the humoral immune responses induced by poly lactide-co-glycolide acid (plga) microspheres coupled inactivated newcastle disease virus (ndv) vaccine in comparison to an ‘in-house’ prepared inactivated and a live commercial vaccine. plg microparticles containing inactivated ndv were prepared by a double emulsion technique based on solvent evaporation ...

Microtechniques are now used extensively in diagnostic as well as immunological studies for viruses, protozoa, and bacteria (Sever, 1962; Edwards, 1964; Kessel, Lewis, Pasquel, and Turner, 1965). In microtitrations constant volumes of fluids are picked up and delivered by specially constructed and calibrated metallic microdiluters. According to the Microtiter Instruction Manual of the Cooke Eng...