نتایج جستجو برای: h2ax

تعداد نتایج: 2027  

Journal: :Molecular biology of the cell 2008
Josée Guirouilh-Barbat Christophe Redon Yves Pommier

The cellular activity of Yondelis (trabectedin, Ecteinascidin 743, Et743) is known to depend on transcription-coupled nucleotide excision repair (TCR). However, the subsequent cellular effects of Et743 are not fully understood. Here we show that Et743 induces both transcription- and replication-coupled DNA double-strand breaks (DSBs) that are detectible by neutral COMET assay and as gamma-H2AX ...

Journal: :Radiation research 2011
Helen C Turner David J Brenner Youhua Chen Antonella Bertucci Jian Zhang Hongliang Wang Oleksandra V Lyulko Yanping Xu Igor Shuryak Julia Schaefer Nabil Simaan Gerhard Randers-Pehrson Y Lawrence Yao Sally A Amundson Guy Garty

The immunofluorescence-based detection of γ-H2AX is a reliable and sensitive method for quantitatively measuring DNA double-strand breaks (DSBs) in irradiated samples. Since H2AX phosphorylation is highly linear with radiation dose, this well-established biomarker is in current use in radiation biodosimetry. At the Center for High-Throughput Minimally Invasive Radiation Biodosimetry, we have de...

Journal: :Clinical cancer research : an official journal of the American Association for Cancer Research 2006
Tatyana A Zykova Feng Zhu Chengrong Lu LeeAnn Higgins Yasuaki Tatsumi Yasuhito Abe Ann M Bode Zigang Dong

PURPOSE Arsenic is a valuable therapeutic tool in cancer treatment. Lymphokine-activated killer T-cell-originated protein kinase (TOPK) is highly expressed in cancer cells, but its specific function is still unknown. We investigated the role of TOPK in arsenic-induced apoptosis in RPMI7951 human melanoma cells. EXPERIMENTAL DESIGN Expression of TOPK was evaluated in different melanoma cell li...

2011
Helen C. Turner David J. Brenner Youhua Chen Antonella Bertucci Jian Zhang Hongliang Wang Oleksandra V. Lyulko Yanping Xu Igor Shuryak Julia Schaefer Nabil Simaan Gerhard Randers-Pehrson Y. Lawrence Yao Sally A. Amundson Guy Garty

The immunofluorescence-based detection of c-H2AX is a reliable and sensitive method for quantitatively measuring DNA double-strand breaks (DSBs) in irradiated samples. Since H2AX phosphorylation is highly linear with radiation dose, this wellestablished biomarker is in current use in radiation biodosimetry. At the Center for High-Throughput Minimally Invasive Radiation Biodosimetry, we have dev...

Journal: :Cancer research 2004
Tom Stiff Mark O'Driscoll Nicole Rief Kuniyoshi Iwabuchi Markus Löbrich Penny A Jeggo

H2AX phosphorylation is an early step in the response to DNA damage. It is widely accepted that ATM (ataxia telangiectasia mutated protein) phosphorylates H2AX in response to DNA double-strand breaks (DSBs). Whether DNA-dependent protein kinase (DNA-PK) plays any role in this response is unclear. Here, we show that H2AX phosphorylation after exposure to ionizing radiation (IR) occurs to similar...

2009
Bu Yin Velibor Savic Marisa M. Juntilla Andrea L. Bredemeyer Katherine S. Yang-Iott Beth A. Helmink Gary A. Koretzky Barry P. Sleckman Craig H. Bassing

The H2AX core histone variant is phosphorylated in chromatin around DNA double strand breaks (DSBs) and functions through unknown mechanisms to suppress antigen receptor locus translocations during V(D)J recombination. Formation of chromosomal coding joins and suppression of translocations involves the ataxia telangiectasia mutated and DNA-dependent protein kinase catalytic subunit serine/threo...

2015
Patrizia Vici Anna Di Benedetto Cristiana Ercolani Laura Pizzuti Luigi Di Lauro Domenico Sergi Francesca Sperati Irene Terrenato Rosanna Dattilo Claudio Botti Alessandra Fabi Maria Teresa Ramieri Lucia Mentuccia Camilla Marinelli Laura Iezzi Teresa Gamucci Clara Natoli Ilio Vitale Maddalena Barba Marcella Mottolese Ruggero De Maria Marcello Maugeri-Saccà

Response of cancer cells to chemotherapy-induced DNA damage is regulated by the ATM-Chk2 and ATR-Chk1 pathways. We investigated the association between phosphorylated H2AX (γ-H2AX), a marker of DNA double-strand breaks that trigger the ATM-Chk2 cascade, and phosphorylated Chk1 (pChk1), with pathological complete response (pCR) in triple-negative breast cancer (TNBC) patients treated with neoadj...

Journal: :International journal of clinical and experimental pathology 2015
Kun Yao Xianzhen Jiang Leye He Yuxin Tang Guangming Yin Qing Zeng Zhiqiang Jiang Jing Tan

Anacardic acid (6-pentadecylsalicylic acid, AA), a natural compound isolated from the traditional medicine Amphipterygiumadstringens, has been reported as potential antitumor agents in various cancers including prostate cancer (PC). However, the effects and mechanism of AA on the radiosensitivity of prostate cancer remains unknown. The results indicated that AA exhibited strong antitumor activi...

H. Zhao, J. Lian, J.J. Chen, L.F. Li, M.Y. Chen, R. Xing, R.Q. Liu, W. Huang, X. Zhou, Y.C. Zeng, Y.Z. Xie,

Background: Tumor radioresistance leads to a reduction in the efficiency of radiation therapy. It is very important to explore the cellular mechanisms leading to radioresistance and to find potential therapeutic targets, which might improve the efficacy of radiation therapy. This study was to investigate the role of ataxia-telangiectasia mutated (ATM) and murine double minute X (MDMX) in radior...

Journal: :Current Biology 2005
Noel F. Lowndes Geraldine W.-L. Toh

How phosphorylated histone H2AX, known as gamma-H2AX, functions in the cellular response to DNA double-strand breaks is the subject of intensive investigation. Recent research in yeast and mammalian cells shows that gamma-H2AX facilitates post-replicational DNA repair by recruiting cohesin, a protein complex that holds sister chromatids together.

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