Dephosphorylation of translation initiation factor 2 (eIF2α) terminates signalling in the mammalian integrated stress response (ISR) and has emerged as a promising target for modifying the course of protein misfolding diseases. The [(o-chlorobenzylidene)amino]guanidines (Guanabenz and Sephin1) have been proposed to exert protective effects against misfolding by interfering with eIF2α-P dephosph...

Guanidine does not inhibit the translation of viral RNA into virus specific proteins but it seems to prevent the generation of functional viral polymerase from precursor material. In the presence of guanidine, "inhibitory protein (s) " responsible for the shut-off of cellular protein synthesis are still produced provided that the multiplicities of infection are high. The incoming (parental) vir...

An asymmetric α-amination of β-keto esters with azodicarboxylate in the presence of a guanidine-bisurea bifunctional organocatalyst was investigated. The α-amination products were obtained in up to 99% yield with up to 94% ee.

A new type of nucleic acid analogues with a phosphoryl guanidine group is described. Oxidation of polymer-supported dinucleoside 2-cyanoethyl phosphite by iodine in the presence of 1,1,3,3-tetramethyl guanidine yields a dinucleotide with an internucleoside tetramethyl phosphoryl guanidine (Tmg) group as the main product. The Tmg group is stable under conditions of solid-phase DNA synthesis and ...

Inhibition of HeLa cell deoxyribonucleic acid (DNA) synthesis, which occurred by the 4th to 5th hr after infection with poliovirus, could be blocked completely by guanidine only when it was present before the 2nd hr. At the 2nd hr, there was no significant ribonucleic acid (RNA)-replicase activity, and addition of guanidine inhibited all production of virus but allowed 57% of maximal DNA inhibi...

In a previous communication, it was shown that on prolonged exposure to urea at temperatures above 20” pepsin is irreversibly inactivated (1). The loss of activity is most marked in the pH range of 4.6 to 5.6 and is always accompanied by the formation of nonprotein material that originates from autodigestion of the protein. Since urea is known to affect the secondary structure of proteins by ru...