Green fluorescent protein (GFP) is autofluorescent. This property has made GFP useful in monitoring in vivo activities such as gene expression and protein localization. We find that GFP can be used in vitro to reveal and characterize protein-protein interactions. The interaction between the S-peptide and S-protein fragments of ribonuclease A was chosen as a model system. GFP-tagged S-peptide wa...

BACKGROUND The behavior of host and graft cells during the healing process after autologous tendon graft has not been elucidated. HYPOTHESIS Host cells will integrate into the bone-tendon interface and contribute to cellular repopulation of the graft. STUDY DESIGN Controlled laboratory study. METHODS Twelve-week-old, genetically identical, female green fluorescent protein transgenic rats ...

Background: Visfatin is protein with insulin-like function that is expressed in high levels in visceral fat tissue and its circulating levels is associated with obesity and diabetes. Objective: The purpose of this study was to examine the effect of 10 weeks of rhythmic aerobic exercise and green tea supplementation on visfatin levels and insulin resistance in obese type II diabetic women. Met...

silver nano-particles have different biological applications due to their compatibility. chemical methodsusually result in remaining some amounts of toxic reactions on the nano-particles. for this reason, the useof plants as sustainable and accessible resources in preparation of compatible nano-particles has attracteda lot of attention in the recent years. this research investigated the green s...

in order to evaluate quantity and quality hairy vetch (vicia villosa l.) yield in the pure and mixed cropping with barley (hordeum vulgare l.) and triticale (triticosecale) the experiment was conducted at agricultural research station (khodabande) under dryland conditions during 2006 -2008. field experiments were arranged in a randomized complete blocks design with 9 treatments in four replicat...

Background: Tumor cells such as leukemia and lymphoma cells are possible targets for gene therapy. However, previously leukemia and lymphoma cells have been demonstrated to be resistant to most of non-viral gene transfer methods. Methods: The aim of this study was to analyze various methods for transfection of primary leukemia cells and leukemia cell lines and to improve the efficiency of gene ...

the herpes simplex virus type 1 (hsv-1) tegument protein, vp22 has been reported to have the property of intercellular transport. the previous studies have shown that following expression of a fusion protein containing vp22 it spreads to every cell in a monolayer and concentrates in the nucleus. in spite of these reports, some studies have shown that vp22 trafficking and its nucleus accumulatio...