نتایج جستجو برای: gra6

تعداد نتایج: 104  

Journal: :The Journal of parasitology 2007
J P Dubey S M Gennari N Sundar M C B Vianna L M Bandini L E O Yai C H Kwok C Suf

The prevalence of Toxoplasma gondii in 118 unwanted dogs from São Paulo City, São Paulo State, Brazil, was determined. Antibodies to T. gondii were assayed by the modified agglutination test and found in 42 (35.8%) dogs, with titers of 1:20 in 10, 1:40 in 6, 1:80 in 5, 1:160 in 5, 1:320 in 6, 1:640 in 7, and 1:1,280 or higher in 3. Hearts and brains of 36 seropositive dogs were bioassayed in mi...

2015
Qing Yin Saeed El-Ashram Hongbin Liu Ximeng Sun Xinxin Zhao Xianyong Liu Xun Suo Sanjai Kumar

Early diagnosis of Toxoplasma gondii infection before the formation of tissue cysts is vital for treatment, as drugs available for toxoplasmosis cannot kill bradyzoites contained in the cysts. However, current methods, such as antibody-based ELISA, are ineffective for detection of early infection. Here, we developed an interferon-gamma release assay (IGRA), measuring the IFN-γ released by T lym...

A. Dalimi, A. Dalir Ghaffari,

Background: Toxoplasmosis, caused by Toxoplasma gondii, is a common parasitic disease, affecting almost one-third of the world’s population. It is transmitted by ingestion of food or water contaminated with oocysts excreted by cats and the consumption of raw or undercooked meat from ruminants. This study aimed at molecular characterization of T. gondii in native cattle from West of Tehran, Iran...

Journal: :The Journal of parasitology 2009
G V Velmurugan C Su J P Dubey

Pigs are considered to be the most important meat source of Toxoplasma gondii for humans in the United States. In the present study, 168 T. gondii isolates (designated TgPgUs15-182) from various sources were genotyped using 10 polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico). Genotyping data ...

2011
Chom-Kyu Chong Wooseog Jeong Hak-Yong Kim Dong-Jun An Hye-Young Jeoung Jeong-Eun Ryu A-Ra Ko Yong-Joo Kim Sung-Jong Hong Zhaoshou Yang Ho-Woo Nam

Rapid serodiagnostic methods for Toxoplasma gondii infection in cats are urgently needed for effective control of transmission routes toward human infections. In this work, 4 recombinant T. gondii antigens (SAG1, SAG2, GRA3, and GRA6) were produced and tested for the development of rapid diagnostic test (RDT). The proteins were expressed in Escherichia coli, affinity-purified, and applied onto ...

Journal: :Veterinary parasitology 2008
G V Velmurugan J P Dubey C Su

Until recently, Toxoplasma gondii was considered to be clonal with very little genetic variability. Recent studies indicate that T. gondii isolates from Brazil are genetically and biologically different from T. gondii isolates from USA and Europe. However, little is known of the genetics of T. gondii strains from Africa. In this study, we genotyped 19 T. gondii isolates from chickens from six A...

Journal: :The Journal of parasitology 2007
J P Dubey H Y López-Torres N Sundar G V Velmurugan D Ajzenberg O C H Kwok R Hill M L Dardé C Su

Cats are essential in the life cycle of Toxoplasma gondii because they are the only hosts that can excrete the environmentally resistant oocysts. Samples of serum, feces, and tissues from cats from Mona, a remote island off the coast of Puerto Rico, were examined for T. gondii infection. Antibodies to T. gondii were assayed by the modified agglutination test and found in 16 of 19 (84.2%) of cat...

2014
Mohammad Talebzadeh Reyhaneh Mohabati Jalal Babaie Samira Amiri Mojgan Allahyari

Toxoplasma gondii is an obligate intracellular protozoan parasite that can infect human and other warm-blooded animals. Although usually asymptomatic in immunocompetent individuals, toxoplasmosis may reactivate in immunocompromised patients (e.g. AIDS patients and organ transplant recipients) and cause severe diseases such as toxoplasmic encephalitis. Moreover, primary maternal infection can pu...

2017
Jamille Rodrigues da Silva Bianca Mendes Maciel Luana Karla Nogueira de Santana Souza Santos Fábio Santos Carvalho Daniele de Santana Rocha Carlos Wilson Gomes Lopes George Rêgo Albuquerque

Strains of Toxoplasma gondii in Brazil are highly genetically diverse compared to strains from North America and Europe. Dogs are epidemiologically important because they act as sentinels for T. gondii infections in humans and are good indicators of environmental contamination. The aim of this study was to isolate and genetically characterize T. gondii strains from tissues of naturally infected...

2015
Renata A. Casagrande Hilda F.J. Pena Aline D. Cabral Veronica M. Rolim Luiz G.S. de Oliveira Fabiana M. Boabaid Angelica T.B. Wouters Flademir Wouters Cláudio E.F. Cruz David Driemeier

An adult, captive raised male Valley quail (Callipepla californica) acquired by a southern Brazilian aviary suddenly showed severe apathy, dyspnea and diarrhea, and died 18 hours after the onset of illness. At necropsy, pale muscles and whitish areas in the heart, splenomegaly, hepatomegaly, and consolidated red lungs were observed. Histological findings were mainly mononuclear inflammation wit...

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