An affinity chromatography system has been developed that retains glycosylated amino acids and peptides. Using this system, synthetic 14C-glycosylated lysine (reduced with NaB3H4) was completely separated from a mixture of reduced 14C-glycosylated lysine and unmodified 3H-lysine. Amino acid analysis of the retained peak from hydrolyzed human diabetic hemoglobin previously reduced with NaB3H4 re...

Conclusions Use of the drug tunicamycin has greatly facilitated study of many different aspects of immunoglobulin biosynthesis. The most striking conclusion is that blocking glycosylation does not prevent synthesis of immunoglobulin heavy chains either directly or by feedback; non-glycosylated heavy chains are able to assemble into immunoglobulin molecules capable of being secreted. Since glyco...

Infections by intracellular pathogens cause significant morbidity and mortality due to lack of efficient drug delivery. Amphotericin B, currently used treat leishmaniasis cryptococcosis, is very toxic cannot eradicate Cryptococcus neoformans (C. neoformans). Glycosylated gold nanoparticles are water dispersible biocompatible with little toxicity. While amphotericin B insoluble in at neutral pH,...

Two New York (NY) strains of the West Nile (WN) virus were plaque-purified and four variants that had different amino acid sequences at the N-linked glycosylation site in the envelope (E) protein sequence were isolated. The E protein was glycosylated in only two of these strain variants. To determine the relationship between E protein glycosylation and pathogenicity of the WN virus, 6-week-old ...

• “Glycoengineering of therapeutic proteins” focusing on how to produce tailored glycosylated versions of monoclonal antibodies as therapeutic proteins. • “Glycoanalytics” focusing on analytical characterization involving methods like mass spectroscopy and chromatographic separation to identify and profile signatures of glycosylated proteins. • “Glycoprotein complex characterization” focusing o...

INTRODUCTION AND OBJECTIVES In patients with heart failure and type 2 diabetes, low glycosylated hemoglobin has been related with higher risk of mortality but information regarding morbidity is scarce. We sought to evaluate the association between glycosylated hemoglobin and 30-day readmission in patients with type 2 diabetes and acute heart failure. METHODS Glycosylated hemoglobin was measur...