The bovine milk protein osteopontin (OPN) may be an efficient means to prevent bacterial adhesion to dental tissues and control biofilm formation. This study sought to determine to what extent OPN impacts adhesion forces and surface attachment of different bacterial strains involved in dental caries or medical device-related infections. It further investigated if OPN's effect on adhesion is cau...

Carbohydrates are characterized by a truly remarkable level of structural diversity which stands in accord with a corresponding functional diversity arguably unsurpassed by any other class of biomolecule. As metabolites, structural components, mediators of molecular interaction, and modifiers of other biomolecules, carbohydrates play central roles in numerous areas of biochemistry. Detailed mol...

Cell-surface glycoconjugates and endogenous lectins have been implicated in cellular interactions that contribute to embryonic development. Functional subsets of primary sensory neurons in mammalian dorsal root ganglia (DRG) have been shown recently to express specific cell-surface oligosaccharide structures. We report here that endogenous lectins with affinity for sensory neuron glycoconjugate...

objective(s):the aim of this study was to evaluate distribution and changes of glycoconjugates of retinal photoreceptors during both pre- and postnatal development. materials and methods: tissue sections from days 15 to 20 of wistar rat embryos and 1 to 12 postnatal days of rat newborns including developing eye were prepared for lectinhistochemistry technique. horseradish peroxidase (hrp)-label...

The growing interest in the synthesis of glycoconjugates is related to the importance of such compounds in various biological processes, including cell-cell recognition and cell-protein interactions. Glycoconjugates may also form the basis of cyclic macromolecules important in molecular recognition . Due to the advances in the Cu(I) 1,3-azide-alkyne cycloaddition (CuAAC), this reaction becomes ...

A long period of studies on carbohydrates such as glycans and carbohydrate moieties of glycoconjugates has brought us a lot of understandings on various biological phenomena in the molecular level. Many of biological phenomena, such as cell-cell recognition, clearance of glycoproteins in blood, infection of pathogens, and initiation of innate immunity, are now recognized as those triggered via ...

Group B Streptococcus (GBS) causes serious infection in neonates and is an important target of vaccine development. Zwitterionic polysaccharides (ZPS), obtained through chemical introduction of positive charges into anionic polysaccharides (PS) from GBS, have the ability to activate human and mouse antigen presenting cells (APCs) through toll-like receptor 2 (TLR2). To generate a polysaccharide...

Using lectin histochemistry, we have previously shown that there are alterations in the distribution of glycoconjugates in the tail bud of chick embryos that parallel the developmental sequence of the caudal axis. If glycoconjugates or the cells bearing them play a role in caudal axial development, then, restriction of their availability by binding with lectins would be expected to produce abno...

The study is oriented at the in vitro evaluation of the immunobiological activity and efficacy of synthetically prepared isomeric pentasaccharides representing fragments of Aspergillus fumigatus cell-wall galactomannan and containing β-(1→5)-linked tetragalactofuranoside chain attached to O-6 (GM-1) or O-3 (GM-2) of a spacer-armed mannopyranoside residue. These compounds were studied as biotiny...