نتایج جستجو برای: glycerophosphate
تعداد نتایج: 936 فیلتر نتایج به سال:
1. Pancreatic islets from several mammalian species were investigated for hydrolytic activity towards glucose 6-phosphate. Both the total phosphatase activity towards this substrate and the proportion cleaving glucose 6-phosphate in preference to beta-glycerophosphate varied widely between species. In pancreatic-islet homogenates prepared from mice and guinea pigs there was a higher rate of lib...
"Null" mutations previously isolated at the alphaGpdh-1 locus of Drosophila melanogaster, because of disruption of the energy-producing alpha-glycerophosphate cycle, severely restrict the flight ability and relative viability of affected individuals. Two "null" alleles, alphaGpdh-1(BO-1-4), and alphaGpdh-1(BO-1-5,) when made hemizygous with a deficiency of the alphaGpdh-1 locus, Df(2L)GdhA, wer...
Enzymatic activity was determined in an optical test, based on the measurement of reduced DPN at 340 rnp in the Beckman spectrophotometer. The reaction was carried out in phosphate or cysteine buffer at pH 7. Synthetic dihydroxyacetone phosphate (5) with reduced DPN or racemic cr-glycerophosphate with DPN was used as substrate to measure the forward and backward reactions, respectively. The con...
Substrate preferences of isolated mitochondria and maximal enzyme activities were used to assess the oxidative capacities of red muscle (RM) and white muscle (WM) of carp (Cyprinus carpio). A 14-fold higher activity of citrate synthase (CS) in RM reflects the higher mitochondrial density in this tissue. RM mitochondria oxidize pyruvate and fatty acyl carnitines (8:O, 12:O, 16:O) at similarly hi...
Activity was determined by the rate of hydrolysis of 13-glycerophosphate on p-nitrophenyl phosphate (8). The determination with sodium 13-glycerophosphate was carried out as follows. The reaction mixture consisted of 0.05 M Tnis-HC1 (pH 10), 0.023 M sodium 13-glycerophosphate, 0.004 M MgCl2, and enzyme in a final volume of 2 ml. After incubation at 37° for 30 mm, 2 ml of 10% tnicbloroacetic ac...
AIMS Medial artery calcification is a common macroangiopathy that initiates from a cell-regulated process similar to osteogenesis. Although the mechanisms governing this process remain unclear, epigenomic regulation by specific microRNAs might play a role in vascular smooth muscle cell (VSMC) calcification. In this study, we aimed to investigate whether miR-204 participates in the regulation of...
Kinetoplastid protozoa compartmentalize the first seven enzymes of glycolysis and two enzymes of glycerol metabolism in a microbody, the glycosome. While in its mammalian host, Trypanosoma brucei depends entirely on glucose for ATP generation. Under aerobic conditions, most of the glucose is metabolized to pyruvate. Aerobic metabolism depends on the activities of glycosomal triosephosphate isom...
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