نتایج جستجو برای: glucokinase

تعداد نتایج: 1803  

Journal: :The Biochemical journal 1991
A J Lange L Z Xu F Van Poelwijk K Lin D K Granner S J Pilkis

Soluble rat liver glucokinase was expressed at high levels at 22 degrees C in the BL21(DE3)pLysS strain of Escherichia coli. Aspartate-211 of yeast hexokinase has been implicated as a catalytic residue from crystallographic data. The corresponding residue in rat liver glucokinase, aspartate-205, was mutated to alanine and the expressed mutant had 1/500th of the activity of the wild type, with n...

Journal: :Biochemical Society transactions 1990
C B Newgard C Quaade S D Hughes J L Milburn

The liver/islet high-K, facilitated glucose transporter (GLUT-2) and the glucose phosphorylating enzyme, glucokinase, are key regulators of the rate of glucose metabolism in liver and islets, the two ‘glucose-sensing’ tissues in mammals. Increases in circulating glucose stimulate insulin secretion from the ,%cells of the islets of Langerhans and convert the liver from an organ o f net glucose o...

Journal: :The Biochemical journal 1966
M J Parry D G Walker

1. An 870-fold purification of glucokinase from rat liver is described which involves ammonium sulphate fractionation and the use of DEAE-Sephadex, DEAE-cellulose and polyacrylamide columns. 2. The preparation is free of any interfering enzymes and has a specific activity of 8mumoles/min./mg. of protein. 3. Glucokinase catalyses the phosphorylation of glucose, mannose and 2-deoxyglucose. 4. The...

Journal: :Current Opinion in Lipidology 2015

Journal: :The Biochemical journal 2008
Loranne Agius

Conversion of glucose into glycogen is a major pathway that contributes to the removal of glucose from the portal vein by the liver in the postprandial state. It is regulated in part by the increase in blood-glucose concentration in the portal vein, which activates glucokinase, the first enzyme in the pathway, causing an increase in the concentration of glucose 6-P (glucose 6-phosphate), which ...

2012
Janne Molnes

.................................................................................................................................... 8 List of publications .................................................................................................................. 10

Journal: :Pharmacological reports : PR 2012
Nirav Dhanesha Amit Joharapurkar Gaurang Shah Vipin Dhote Samadhan Kshirsagar Rajesh Bahekar Mukul Jain

Exendin-4 is a stable peptide agonist of GLP-1 receptor that exhibits insulinotropic actions. Some in vivo studies indicated insulin-independent glucoregulatory actions of exendin-4. That finding prompted us to evaluate effects of exendin-4 on liver glucose metabolism. Acute and chronic treatment of exendin-4 resulted in increased hepatic glucokinase activity in db/db mice but not in lean C57 m...

2013
Jun Shirakawa Yu Togashi Eri Sakamoto Mitsuyo Kaji Kazuki Tajima Kazuki Orime Hideaki Inoue Naoto Kubota Takashi Kadowaki Yasuo Terauchi

The derangement of endoplasmic reticulum (ER) homeostasis triggers β-cell apoptosis, leading to diabetes. Glucokinase upregulates insulin receptor substrate 2 (IRS-2) expression in β-cells, but the role of glucokinase and IRS-2 in ER stress has been unclear. In this study, we investigated the impact of glucokinase activation by glucokinase activator (GKA) on ER stress in β-cells. GKA administra...

Journal: :The Biochemical journal 1990
D J Hayzer P B Iynedjian

The sequences of two near full-length cDNAs encoding rat liver glucokinase are reported. One of the cDNAs is essentially identical to the cDNA cloned by Andreone, Printz, Pilkis, Magnuson & Granner. [(1989) J. Biol. Chem. 264, 363-369]. The other cDNA contains a 151 bp insertion and a downstream 52 bp deletion. The inserted block of bases has been shown to originate from an optional cassette ex...

2017
Antasar Ali D. Claire Wathes Angelina Swali Helena Burns Shamus Burns

BACKGROUND Hexokinase and glucokinase enzymes are ubiquitously expressed and use ATP and ADP as substrates in mammalian systems and a variety of polyphosphate substrates and/or ATP in some eukaryotic and microbial systems. Polyphosphate synthesising or utilizing enzymes are widely expressed in microbial systems but have not been reported in mammalian systems, despite the presence of polyphospha...

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