Kubota Y, Putkey JA, Shouval HZ, Waxham MN. IQ-motif proteins influence intracellular free Ca in hippocampal neurons through their interactions with calmodulin. J Neurophysiol 99: 264–276, 2008. First published October 24, 2007; doi:10.1152/jn.00876.2007. Calmodulin (CaM) is most recognized for its role in activating Ca –CaMdependent enzymes following increased intracellular Ca . However, CaM’s...

Ca(2+) rise and nitric oxide (NO) generation are essential early steps in plant innate immunity and initiate the hypersensitive response (HR) to avirulent pathogens. Previous work from this laboratory has demonstrated that a loss-of-function mutation of an Arabidopsis (Arabidopsis thaliana) plasma membrane Ca(2+)-permeable inwardly conducting ion channel impairs HR and that this phenotype could...

Ca rise and nitric oxide (NO) generation are essential early steps in plant innate immunity and initiate the hypersensitive response (HR) to avirulent pathogens. Previous work from this laboratory has demonstrated that a loss-of-function mutation of an Arabidopsis (Arabidopsis thaliana) plasma membrane Ca-permeable inwardly conducting ion channel impairs HR and that this phenotype could be resc...

Among the multiple roles assigned to calmodulin (CaM), controlling the surface expression of Kv7.2 channels by binding to two discontinuous sites is a unique property of this Ca(2+) binding protein. Mutations that interfere with CaM binding or the sequestering of CaM prevent this M-channel component from exiting the endoplasmic reticulum (ER), which reduces M-current density in hippocampal neur...

The Anti-angiogenic Activity of Phoenix dactylifera seeds methanol extract in vivo study Abstract: The aim of the study was to identify the antiangiogenic activity of Phoenix dactylifera seeds methanolic extract in vivo and the probable anti-angiogenic mechanism of action. The date seeds were extracted with methanol using cold method, the crude extract was tested on chick chorioallantoic ...

Lipid oxidation products, including lysophosphatidylcholine (lysoPC), activate canonical transient receptor potential 6 (TRPC6) channels leading to inhibition of endothelial cell (EC) migration in vitro and delayed EC healing of arterial injuries in vivo. The precise mechanism through which lysoPC activates TRPC6 channels is not known, but calmodulin (CaM) contributes to the regulation of TRPC ...

Calmodulin (CaM) and phosphatidylinositide-3 kinase (PI3Kα) are well known for their multiple roles in a series of intracellular signaling pathways and in the progression of several human cancers. Crosstalk between CaM and PI3Kα has been an area of intensive research. Recent experiments have shown that in adenocarcinoma, K-Ras4B is involved in the CaM-PI3Kα crosstalk. Based on experimental resu...

Orai1 is a plasma membrane protein that in its tetrameric form is responsible for calcium influx from the extracellular environment into the cytosol in response to interaction with the Ca(2+)-depletion sensor STIM1. This is followed by a fast Ca(2+)·calmodulin (CaM)-dependent inhibition, resulting from CaM binding to an Orai1 region called the calmodulin binding domain (CMBD). The interaction b...

BACKGROUND Many targets of calcium signaling pathways are activated or inhibited by binding the Ca(2+)-liganded form of calmodulin (Ca(2+)-CaM). Here, we test the hypothesis that local Ca(2+)-CaM-regulated signaling processes can be selectively activated by local intracellular differences in free Ca(2+)-CaM concentration. RESULTS Energy-transfer confocal microscopy of a fluorescent biosensor ...