Time-of-flight secondary ion mass spectrometry (TOF-SIMS) is a well-established bioanalytical method for directly imaging the chemical distribution across single cells. Here we report a protocol for the use of SIMS imaging to comparatively quantify the relative difference in cholesterol level between the plasma membranes of two cells. It should be possible to apply this procedure to the study o...

Single-molecule speckle (SiMS) microscopy has been a powerful method to analyze actin dynamics in live cells by tracking single molecule of fluorescently labeled actin. Recently we developed a new SiMS method, which is easy-to-use for inexperienced researchers and achieves high spatiotemporal resolution. In this method, actin labeled with fluorescent DyLight dye on lysines is employed as a prob...

PURPOSE In the mid 1800s Dr. J. Marion Sims reported the successful repair of vesicovaginal fistulas with a technique he developed by performing multiple operations on female slaves. A venerated physician in his time, the legacy of Dr. Sims is controversial and represents a significant chapter in the mistreatment of African-Americans by the medical establishment. This review compares the modern...

We present a novel method for correlating and classifying ion-specific time-of-flight secondary ion mass spectrometry (ToF-SIMS) images within a multispectral dataset by grouping images with similar pixel intensity distributions. Binary centroid images are created by employing a k-means-based custom algorithm. Centroid images are compared to grayscale SIMS images using a newly developed correla...

Purpose – Security information management systems (SIMs) have been providing a unified distributed platform for the efficient management of security information produced by corresponding mechanisms within an organization. However, these systems currently lack the capability of producing and enforcing response policies, mainly due to their limited incident response (IR) functionality. This paper...

In recent years, there has been a growing interest in computer graphics and geometric modeling in the ability to create and manipulate Seemingly Impossible Models (SIMs). Methods to create derivative work and modify drawings and paintings of SIMs made by artists were suggested. Similarly, 3D realization of SIMs of some of these drawings were also offered. In this work, we further explore the na...

The nondestructive nature of static secondary ion mass spectrometry (SIMS) in the context of studies of self-assembled monolayers (SAMs) of organic molecules has been examined by measuring the primary ion fluence dependence of secondary ion signals with two well-known SAMs, C18H37SH on Au(1 1 1) and C18H37PO3H2 on freshly cleaved mica. This SIMS analysis is challenging because the bonding natur...

BACKGROUND Modification of proteins by the small ubiquitin like modifier (SUMO) is an essential process in mammalian cells. SUMO is covalently attached to lysines in target proteins via an enzymatic cascade which consists of E1 and E2, SUMO activating and conjugating enzymes. There is also a variable requirement for non-enzymatic E3 adapter like proteins, which can increase the efficiency and s...

Freeze-fracture techniques have been used to maintain chemical heterogeneity of frozen-hydrated mammalian cells for static TOF-SIMS imaging. The effects the fracture plane has on scanning electron microscopy and dynamic SIMS images of cells have been studied, but the implications this preparation method has on static SIMS have not been addressed to date. Interestingly, the chemical specificity ...

diffusion of nitrogen in plasma nitrided iron and structural evolution during the nitriding process were evaluated by several characterization techniques including optical microscopy (om), microhardness depth profiling (hdp), scanning electron microscopy (sem), x-ray diffraction (xrd), glow discharge optical emission spectroscopy (gdoes), and secondary ion mass spectroscopy (sims). plasma nitri...