In this paper, we compare four field-of-view (FOV) metrics that, when applied to a low-resolution image of a microscope slide, are capable of both accurately delineating the specimen and selecting a subset of focus candidate FOVs required for construction of high-resolution focus map. The metrics evaluated are: threshold index (TI) that measures image intensity; normalised auto-correlation inde...

METHODS All State TB control programmes in Nigeria were requested to submit 25-50 smear-positive Ziehl-Neelsen (ZN) stained slides for screening during 2013-2014. DNA was extracted from 929 slides for spoligotyping and drug-resistance analysis using microbead-based flow-cytometry suspension arrays. RESULTS Spoligotyping results were obtained for 549 (59.1%) of 929 samples. Lineage 4 Cameroon ...

background: hirschsprung disease is a complex genetic disorder of the enteric nervous system (ens), often called congenital aganglionic megacolon and characterized by the absence of enteric neurons along a variable length of the intestine. the definitive diagnosis of hirschsprung disease relies on histologic and/or histochemical staining of sections from suction rectal biopsies. calretinin immu...

Methodology: In this hospital based case-control study, 22 subjects (11 cases of lip neoplasm and 11 healthy individuals as Control group) were included in this study. Scraped exfoliated cytosmears were collected from the affected site of the lip and smearing was done in the precleaned-coded glass-slides. Two such slides were prepared from each subject. The cytosmears were immediately fixed in ...

BACKGROUND The majority of thyroid nodules are diagnosed using fine-needle aspiration (FNA) biopsies. The authors recently described the clinical validation of a molecular microRNA-based assay, RosettaGX Reveal, which can diagnose thyroid nodules as benign or suspicious using a single stained FNA smear. This paper describes the analytical validation of the assay. METHODS More than 800 FNA sli...

We here propose a simple and fast hands-on activity requiring limited equipment, to introduce students of various levels (bachelor’s or high school) backgrounds (from science arts) the chemistry color. The different colors stained glass are replicated through deposition, on ordinary slides, silica coatings colored by addition metal ions nanoparticles. A sol is used as matrix embed ions, followe...

Abstract Mohs micrographic surgery (MMS) relies on good-quality, well-stained frozen sections for effective histological analysis. Slides should also be produced as quickly possible. Automation of complementary devices in the laboratory ensures standardization and efficiency improves overall quality performance. Our study evaluated performance Autostainer-XL (Leica) vs. Linistat (Thermo Scienti...

Smears of fine-needle aspiration (FNA) biopsy specimens of 24 histologically proven and MIB-1– positive hyalinizing trabecular adenomas stained with Papanicolaou or Diff-Quik were stripped from the original slides, transferred to charged slides, destained, and restained with MIB-1. Positive immunoreactivity with MIB-1 in a peripheral cytoplasmic pattern was present in 17 specimens, no immunorea...