An increase in frequency of administration of exogenous growth hormone (GH) or GH-releasing hormone was reported to be a model to increase blood circulating insulin-like growth factor-1 (IGF-1) and to improve growth performance in animals. We have investigated the effect of twice daily administration of GH-releasing peptide-2 (GHRP-2) on growth performance, GH responsiveness and plasma insulin-...

The importance of gender-specific growth hormone (GH) secretion pattern in the regulation of growth and metabolism has been demonstrated clearly in rodents. We recently showed that GH secretion in humans is also sexually dimorphic. Whether GH secretion pattern regulates the metabolic effects of GH in humans is largely unknown. To address this question, we administered the same daily intravenous...

GH and GH receptors are expressed throughout life, and GH elicits a diverse range of responses, including growth and altered metabolism. It is therefore important to understand the full spectrum of GH signaling pathways and cellular responses. We applied mass spectrometry-based phosphoproteomics combined with stable isotope labeling with amino acids in cell culture to identify proteins rapidly ...

A sensitive immunoradiometric assay (IRMA) for human growth hormone (GH) using monoclonal antibodies was evaluated for clinical application. The detection limit of GH in serum was 0.05 ng/ml using 0.1 ml of serum. The specificity and assay precision were satisfactory. GH levels in serum determined by the IRMA were well correlated to those determined by the sensitive enzyme immunoassay or the co...

It has been suggested that (abdominally) obese individuals are hypersensitive to growth hormone (GH) action. Because GH affects glucose metabolism, this may impact glucose homeostasis in abdominal obesity. Therefore, we studied the effect of GH on glucose metabolism in abdominally obese (OB) and normal-weight (NW) premenopausal women. A 1-h intravenous infusion of GH or placebo was randomly adm...

Objective: Growth hormone (GH)-releasing hormone (GHRH) and GH-releasing peptides (GHRPs) stimulate the release of GH through their specific receptors on somatotropes. Combined GHRH and GHRP administration causes a synergistic GH release in vivo by an unknown mechanism. The current study focuses on the direct action of GHRH and GHRP on several molecular targets in somatotropes. Design and metho...

GH mediates most of its effects by releasing insulin-like growth factor-1 (IGF-1) through stimulation of GH receptors (GHR), in particular in the liver. Both GH and IGF-1 are shown to cross the blood-brain barrier (BBB) and affect specific targets for these hormones in the brain. The discovery of GH and IGF-1 receptors in the CNS and their distribution in brain areas associated with the functio...

BACKGROUND Release of growth hormone (GH), putatively through alterations in insulin growth factor-1 (IGF-1) levels, has been implicated to influence left ventricular (LV) myocardial structure and function. The objective of this study was to determine contributory mechanisms by which GH supplementation may influence LV function with the development of congestive heart failure (CHF). METHODS A...

Antagonists of growth hormone-releasing hormone (GH-RH) inhibit growth of various human cancers including osteosarcomas and Ewing's sarcomas, xenografted into nude mice or cultured in vitro. The antiproliferative effect of GH-RH antagonists could be mediated, in part, through the splice variants (SVs) of receptors for GH-RH which have been found in several human cancers and cancer cell lines. I...

This investigation aimed to study polymorphism of growth hormone (GH), growth hormone receptor (GHR), and insulin like growth factor 1 (IGF-1) genes and their associations with growth and carcass traits in Mazandaran native chicken. 200 male chicks were reared and slaughtered at 12 weeks of age. Traits including live weight at 4, 8 and 12 weeks of age, body weight and weights of heart, liver, g...