the potential mycoherbicide alternaria alternata was isolated from one lesion on a leaf of amaranthus retroflexus which had been inoculated with conidia of the fungus. the isolate was cultured on v-8 agar medium. hyphal tip isolates (hti) were derived from young germ tubes of single spores and cultured on a semidefined culture medium with a c:n ratio of 15:1. amplified fragment length polymorph...

Germ tubes of Uromyces fabae differentiated into appressoria which were deliminated either by one or two septa. These appressoria developed rapidlyon artificial membranes as well as on leaf surfaces. Fluorescein-conjugated wheat germ aggJutinin (WGA) bound strongly tO the germ tube walls of U. fabae and its binding ability did not differ significantly in 3, 5 and 7 hold germ tubes. The binding ...

Pax-3 is a transcription factor that is expressed in the neural tube, neural crest, and dermomyotome. We previously showed that apoptosis is associated with neural tube defects (NTDs) in Pax-3-deficient Splotch (Sp/Sp) embryos. Here we show that p53 deficiency, caused by germ-line mutation or by pifithrin-alpha, an inhibitor of p53-dependent apoptosis, rescues not only apoptosis, but also NTDs,...

Germ tubes of Candida albicans and C. stellatoidea clump in normal serum but disperse in serum from animals infected with either species of Candida. A new procedure for the assessment of grades of germ tube dispersion activity of serum is presented; this procedure is to count the number of freely dispersed germ tubes in test serum into which a definite number of yeast-type C. albicans has been ...

To identify proteins synthesized during blastospore to germ tube transformation in Candida albicans, membrane and cytoplasmic protein fractions labelled with 14C were analysed by SDS-PAGE and autoradiography. Four cytoplasmic proteins were detected in pulse-labelled lysates prepared from cells forming blastospores and germ tubes at 37 degrees C, but not in pulse-labelled lysates prepared from c...

A new medium composed of "cream of rice" infusion, oxgall, Tween 80, and agar is described for the sequential development of germ tubes and chlamydospores by Candida albicans. The procedure used (Dalmau's technique) is an improvement over the fluid substrate procedures previously advocated for germ tube formation. That the same preparation is then used for chlamydospore production is of practic...

The effect of activated lansoprazole (AG 2000), a novel benzimidazole proton pump inhibitor, against hypha formation of Candida albicans was examined in hypha-forming medium pH 7 (HFM7) after 20 h. AG 2000, at 50-800 microM, did not inhibit germ tube formation. However, it inhibited elongation of germ tubes to form hyphae and favored conversion of germ tubes to resume yeast growth at concentrat...

The goal of this study was to determine the time to detection of Candida species isolates using the two most commonly used automated blood culture systems, and to evaluate rapid, widely available methods for the presumptive identification of C. albicans. Candidemia models of eight commonly detected Candida species were prepared using ATCC standards. The times to detection were evaluated using t...

Elevated irradiance has a profound effect on the successful dispersal and establishment of kelp zoospores, affecting their physiology and viability. The research to date, however, has been on zoospores localized near the benthos, with little attention on the importance of vertical transportation and subsequent exposure to increased irradiance. Therefore, we wanted to investigate the effects of ...