نتایج جستجو برای: genotyping techniques

تعداد نتایج: 650504  

2015
Chao-Wei Huang Yu-Tsung Lin Shih-Torng Ding Ling-Ling Lo Pei-Hwa Wang En-Chung Lin Fang-Wei Liu Yen-Wen Lu

The genetic markers associated with economic traits have been widely explored for animal breeding. Among these markers, single-nucleotide polymorphism (SNPs) are gradually becoming a prevalent and effective evaluation tool. Since SNPs only focus on the genetic sequences of interest, it thereby reduces the evaluation time and cost. Compared to traditional approaches, SNP genotyping techniques in...

2011
Marcelo Miyata Adolfo Carlos Barreto Santos Natália Helena Mendes Eunice Atsuko Cunha Fernando Augusto Fiúza de Melo Clarice Queico Fujimura Leite

We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.

Journal: :Gematologiia i transfuziologiia 2021

Introduction . Blood transfusion is a strong practice in traumatology, internal medicine, haematology, obstetrics and transplantation, which demands safety of haemotransfusion with estimating the red blood cell group antigens donor recipient blood. Routine immunotyping techniques usually provide for an antigen identification to weak subgroups, albeit certain inherent limitations serology tests ...

Journal: :Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing 2004
Roded Sharan Amir Ben-Dor Zohar Yakhini

A generic genotyping assay utilizes a fixed set of reagents, which is independent of the actual target sample, to determine all present alleles. An example is the interrogation of several amplicons spanning polymorphic sites using an all k-mer array. Due to the high cost associated with a genotyping experiment, it is desirable to design a set of experiments, which maximizes the number of SNPs t...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه زنجان - دانشکده علوم انسانی و اجتماعی 1392

most specialists in the field of foreign language teachingconsiderreading skill as an interactive process between the reader’s prior knowledge and the text.accordingly, the activation of prior knowledge for an effective comprehension is very important. it is generally agreed that the pre-reading phase is the stage where this type of interaction and activation may be enhanced throughcertain stra...

2010
Yan Fu Nathan M. Springer Kai Ying Cheng-Ting Yeh A. Leonardo Iniguez Todd Richmond Wei Wu Brad Barbazuk Dan Nettleton Jeff Jeddeloh Patrick S. Schnable

To date, microarray-based genotyping of large, complex plant genomes has been complicated by the need to perform genome complexity reduction to obtain sufficiently strong hybridization signals. Genome complexity reduction techniques are, however, tedious and can introduce unwanted variables into genotyping assays. Here, we report a microarray-based genotyping technology for complex genomes (suc...

Journal: :Genetics and molecular research : GMR 2016
U R Pagel R S Reis V P Carvalho E V W Santos E Zandonade I D Louro F Paula

Short tandem repeats (STRs) are commonly used as genetic markers. The detection and analysis of STRs can be used to gather information on polymorphisms of interest to forensic geneticists. Denaturing polyacrylamide gel electrophoresis (PAGE) is an affordable method for the detection of minor sequence changes in DNA, while capillary electrophoresis (CE) is the gold standard for genotyping analys...

2009
Alison M. Dunning Craig Luccarini

The new field of genomics has emerged from conventional human genetics and is, at present, moving extremely rapidly. As a result, this chapter will be a snapshot in time, capturing a moment in the state of development. For this reason it will not dwell in detail on individual technologies or machines, as many of these will be obsolete in a very short time, but rather on the principles of high-t...

2012
Katarzyna Wolska Piotr Szweda

Typing of microbial pathogens, or identifying bacteria at the strain level, is particularly important for diagnosis, treatment, and epidemiological surveillance of bacterial infections. This is especially the case for bacteria exhibiting high levels of antibiotic resistance or virulence, and those involved in nosocomial or pandemic infections. Strain typing also has applications in studying bac...

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