cloning and expression of the l-phenylalanine dehydrogenase gene, from b. sphaericus in e. coli were done. the gene was cloned in the vector pet16b and transformed into e. coli bl21 (de3). the functional form of the l-phenylalanine dehydrogenase enzyme was purified by affinity purification techniques, taking advantage of the ability of this enzyme to bind to the nucleotide site affinity dye, re...

Microsatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and 65 clones were screened for usable microsatellites, of which 36 were deemed unique, of sufficient length (more than 8 repeats) and possessed adequate flanking regions for primer designment. Among 36 pri...

Microsatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and 65 clones were screened for usable microsatellites, of which 36 were deemed unique, of sufficient length (more than 8 repeats) and possessed adequate flanking regions for primer designment. Among 36 pri...

hepatitis c virus (hcv) encodes two envelope glycoproteins, e1 and e2. our previous work selected a specific aptamer ze2, which could bind to e2 with high affinity, with a great potential for developing new molecular probes as an early diagnostic reagents or therapeutic drugs targeting hcv. in this study, the binding sites between e2 and aptamer ze2 were further explored. e2 was truncated to 15...

Previous work has shown that the GroEL-GroES interaction is primarily mediated by the GroES mobile loop. In bacteriophage T4 infection, GroES is substituted by the gene 31-encoded cochaperonin, Gp31. Using a genetic selection scheme, we have identified a new set of mutations in gene 31 that affect interaction with GroEL; all mutations result in changes in the mobile loop of Gp31. Biochemical an...

Garden escapes of Acer campestre spread as a cryptic invading gene pools and challenge the conservation unique ancient population in southern Scandinavia. The native pool consists just 34 individuals is listed critically endangered. This more than 150 years old represents diorama into an almost extinct genetic diversity early 19th century. That cannot be separated from introduced by morphology ...

Glucagon-like peptide-1 (GLP-1) is considered to be a promising peptide for the treatment of type 2 diabetes mellitus (T2DM). However, extremely short half-life GLP-1 limits its clinical application. Albumin-binding domain (ABD) with high affinity human serum albumin (HSA) has been used widely extension therapeutic peptides and proteins. In present study, novel receptor agonists were designed b...

The agonistic potentials of therapeutic anti-CD40 antibodies have been profiled in relation to antibody isotype and epitope specificity. Still, clinical impact relies on a well-balanced efficacy versus target-mediated toxicity. As CD40-mediated immune activation must rely combination stimulation antigen-presenting cells (APCs) alongside antigen presentation, for efficient T cell priming, altern...

A new technology, genetic alphabet expansion using artificial bases (unnatural bases), has created high-affinity DNA ligands (aptamers) that specifically bind to target proteins by ExSELEX (genetic alphabet Expansion for Systematic Evolution of Ligands by EXponential enrichment). We recently found that the unnatural-base DNA aptamers can be stabilized against nucleases, by introducing an extrao...