Transcriptional pulsing has been observed in both prokaryotes and eukaryotes and plays a crucial role in cell-to-cell variability of protein and mRNA numbers. An important issue is how the time constants associated with episodes of transcriptional bursting and mRNA and protein degradation rates lead to different cellular mRNA and protein distributions, starting from the transient regime leading...

MOTIVATION Alternative splicing has the potential to generate a wide range of protein isoforms. For many computational applications and for experimental research, it is important to be able to concentrate on the isoform that retains the core biological function. For many genes this is far from clear. RESULTS We have combined five methods into a pipeline that allows us to detect the principal ...

Romain Yvinec ∗ and Alexandre F. Ramos † Institut Camille Jordan UMR 5208 Université Claude Bernard Lyon 1 43 Bd du 11 novembre 1918 69622 Villeurbanne cedex France Escola de Artes, Ciências e Humanidades, Universidade São Paulo, Av. Arlindo Béttio, 1000, CEP. 03828-000, São Paulo, SP, Brazil ‡ Abstract In this article we demonstrate that the so-called bursting production of molecular species d...

As the sequencing of the total DNA of many organisms continues, attention is turning next to the interpretation of the function of all of the genes and gene products, with the aim of learning the full meaning of the entire genetic blueprint of a sequenced organism in concrete terms. To set the stage for accomplishing this, systems need to be constructed for the expression of the functions of ge...

Gene products required for mitotic chromosome separation in the fission yeast Schizosaccharomyces pombe are described. They have been identified by two distinct strategies of mutant isolation, followed by gene cloning and immunochemical characterization of gene products. The roles of four representative genes, namely nda3+, nuc2+, top2+ and dis2+, encoding beta-tubulin, a nuclear scaffold-like ...

Similar to other herpesviruses, human cytomegalovirus remains in the infected host following resolution of the primary infection. The ability to persist in the host after primary infection is believed to be strongly influenced by the ability of HCMV to down-regulate immune recognition of infected cells. One of the genes contributing to immune evasion is the US3 gene. The US3 gene has been shown...

Regulation of apoptosis by viral gene products.

Many disease genes encode proteins that are difficult to target directly using small molecule drugs. Improvements in libraries based on synthetic compounds, natural products, and other types of molecules may ultimately allow some challenging proteins to be successfully targeted; however, these developments alone are unlikely to be sufficient. A complementary strategy exploits the functional int...