n the present work, isolation and identification of some plant beneficial bacteria vermicompost were conducted. A total ten bacterial isolates selected characterized morphologically biochemically. Molecular characterization using 16S rRNA gene sequence analysis phylogenic tree According to percentages homology with closest NCBI strain, all belonged six species (

background: campylobacter jejuni and campylobacter coli are zoonotic bacteria which are frequently associated with human diarrhea. sharing of the cytolethal distending toxin (cdt) genes in campylobacter is common and is considered species specific. objectives: in this study we focused on detecting the presence of cdt gene in c. jejuni and c. coli isolated from broilers, turkeys and quails of ir...

The biosynthetic pathways of saturated and unsaturated fatty acids consist of many steps controlled by various enzymes. One of the methods for improving oil quality is to change the fatty acid profile through genetic manipulation which requires isolation and characterization of the genes and other cis-acting elements, such as the promoter, involved in fatty acid biosynthesis. b-ketoacyl-CoA syn...

Patterns of reproductive isolation between species may provide insight into the mechanisms and evolution of barriers to interspecific gene exchange. We used data from published interspecific hybridization experiments from 14 genera of angiosperms in order to test for the presence of asymmetrical barriers to gene exchange. Reproductive isolation was examined at three life-history stages: the abi...

objective: in this study, the possibility of prenatal diagnosis of down syndrome with real-time pcr method was evaluated. in this context, optimization of a suitable method for purification of high quality dna from amniotic fluid samples was also considered. materials and methods: pregnant women who had the high risk of having babies with down syndrome were selected according to the biochemica...

The amount of sequence data available today highly facilitates the access to genes from many gene families. Primers amplifying the desired genes over a range of species are readily obtained by aligning conserved gene regions, and laborious gene isolation procedures can often be replaced by quicker PCR-based approaches. However, in the case of multigene families, PCR-based approaches bear the of...

Background: Asthma is very common in children and its diagnosis is based on clinical manifestations, which can be misdiagnosed as other respiratory diseases with similar signs and symptoms. Objective: To analyze the expression of ST2L and CD203c in the diagnosis of pediatric asthma. Methods: Basophils were purified from whole blood samples of patients and healthy controls using Ficol-Paque grad...

Drosophila simulans and Drosophila mauritiana are sibling species that show substantial sexual isolation in one of their two reciprocal hybridizations. Genetic analysis reveals that in females this isolation is caused by at least one recessive gene on each autosome, while the X chromosome has little or no effect. Our results, combined with those of previous studies, show that in Drosophila the ...

Background: The isolation of the TEM gene in Pseudomonas aeruginosa and Escherichia coli and the antibiotic resistance pattern provides useful information on the epidemiology and factors involved in these infections. Aim: The aim of this study was to evaluate antibiotic resistance and abundance of a beta-lactamase gene (TEM) in P.aeruginosa and E.coli isolated from clinical specimens by the po...