The microbiota of four individual deep water sponges, Lissodendoryx diversichela, Poecillastra compressa, Inflatella pellicula, and Stelletta normani, together with surrounding seawater were analysed by pyrosequencing of a region of the 16S rRNA gene common to Bacteria and Archaea. Due to sampling constraints at depths below 700 m duplicate samples were not collected. The microbial communities ...

Using ammonium bicarbonate (AB) particles as a porogen, chitosan (CS)-based hemostatic porous sponges were prepared in supercritical carbon dioxide due to its low viscosity, small surface tension, and good compatibility with organic solvent. Fourier transform infrared spectroscopy (FTIR) spectra demonstrated that the chemical compositions of CS and poly-(methyl vinyl ether-co-maleic anhydride) ...

The dichotomy between high microbial abundance (HMA) and low microbial abundance (LMA) sponges has been observed in sponge-microbe symbiosis, although the extent of this pattern remains poorly unknown. We characterized the differences between the microbiomes of HMA (n = 19) and LMA (n = 17) sponges (575 specimens) present in the Sponge Microbiome Project. HMA sponges were associated with richer...

Objective: A randomized, controlled trial was performed to evaluate a computer-assisted method for counting sponges using a barcode system. Background: Retained sponges are a rare and preventable problem but persist in surgery despite standardized protocols for counting. Technology that improves detection of counting errors could reduce risk to surgical patients. Methods: We performed a randomi...

reference taxon for studying metazoan origins. Like a living time capsule, they may allow us to infer the genome content and cell biology of the unicellular progenitors of Metazoa. For example, comparing choanoflagellate and metazoan genomes will reveal the minimal genetic toolkit of the first metazoan. Pilot studies of choanoflagellate expressed genes have already uncovered homologs of a remar...

Highly porous collagen-glycosaminoglycan (CG) sponges, ultimately to be used as cell-seeded implants to facilitate the regeneration of selected soft tissues, were synthesized and cross-linked to various degrees using both chemical (ethanol, glutaraldehyde) and physical (dehydrothermal, ultraviolet irradiation) methods. Tendon fibroblasts were seeded into the scaffolds and the contraction of the...

background: development of biodegradable and biocompatible films based on protein polymer with strong antioxidant and antibacterial activities has gradually obtained extensive concern in the world. in this study, the improvement of gelatin film properties incorporated with ferula assa-foetida essential oil (fao) as a potential antioxidant/antibacterial wound dressing film was investigated. mate...

OBJECTIVES/HYPOTHESIS Artificial tracheas prepared using a collagen sponge and polypropylene mesh have been implanted in patients who received tracheal resections, but epithelialization in the reconstructed area is slow. We determined the optimal bovine atelocollagen concentration necessary for the rapid and complete tracheal epithelial coverage of collagen sponge implants. STUDY DESIGN Preli...

Total amounts and patterns of bromoisoxazoline alkaloids of Aplysina sponges from Croatia (Mediterranean Sea) were analyzed along an underwater slope ranging from 1.8 to 38.5 m. Total amounts of alkaloids varied from sample to sample and showed no correlation with depth. In contrast, striking differences of alkaloid patterns were found between sponges from shallow sites (1.8-11.8 m) and those c...