Gel-free proteomics has emerged as a complement to conventional gel-based proteomics. Gel-free approaches focus on peptide or protein fractionation, but they do not address the efficiency of protein processing. We report the development of a microfluidic proteomic reactor that greatly simplifies the processing of complex proteomic samples by combining multiple proteomic steps. Rapid extraction ...

this study was conducted to investigate the effect of physical and chemical processing on crude protein fractions of two varieties of canola (okapi and modena) and safflower (62 and zarfan) seed using cornel net carbohydrate and protein system and on protein subunits using sodium dodecyl sulfate polyacrylamide gel electrophoresis. formaldehyde was used as chemical treatment, while toasting and ...

0

Two-dimensional gel electrophoresis (2-DE) separation has not been considered suitable for large-scale comparative protein expression studies due to its limited throughput. We present a high-throughput analysis method based on three-dimensional (3-D) geometry gel electrophoresis. Following conventional isoelectric focusing (IEF), up to 36 immobilized pH gradient (IPG) strips are arrayed on the ...

Aloe vera gel is widely used as a functional ingredient in food processing. This gel is very unstable due to its high water activity. Drying process can be used to obtain shelf stable products. Response surface methodology was used to assess the effects of solution concentration (30-60% w/w), solution temperature (30-50°C) and immersion time (4-6h) at atmospheric pressure and constant solution ...

Proteolytic digestion is an essential step in proteomic sample processing. While this step has traditionally been implemented in homogeneous (solution) format, there is a growing trend to use heterogeneous systems in which the enzyme is immobilized on hydrogels or other solid supports. Here, we introduce the use of immobilized enzymes in hydrogels for proteomic sample processing in digital micr...

Introduction: Polymer gel dosimeters contain chemical materials sensitive to the radiation which are polymerized by the radiation as a function of absorbed dose. So information of spatial dose distribution can be extracted by imaging from irradiated gel. Among imaging techniques, computed tomography (CT) poses as an attractive method because of practical advantages such as acce...

the synthesis of titania via sol-gel method has been widely studied. in the present work, titania was deposited onto a titanium substrate via sol-gel method. different processing parameters such as the repetition number of dipping/heat treatment process and dipping time have been studied to obtain the homogenous titania coating. phase and microstructural evaluation of the powder and coating sam...

Ridge and buried channel waveguides were fabricated on different substrates such as SiO2-on-Si, Si, glass wafers and Printed Circuit Boards by a sol-gel processing together with a photolithography process using photopatternable hybrid sol-gel materials. Thick sol-gel films of up to 100 microns were developed using a modified sol-gel process and ridge waveguides with vertical smooth sidewalls we...

Introduction 2 Deoxyribonucleic Acid to Ribonucleic Acid 4 Ribonucleic Acid to Protein 6 Proteome Handling in Classical and Functional Proteomics 6 Protein Purification 6 Cell Culture 8 Other Source of Samples 8 Subpopulation of Cells 10 Subcellular Components 11 Protein Separation by Two-Dimensional Gel Electrophoresis 12 Principles of Two-Dimensional Gel Electrophoretic Separation 13 Detectio...