Pair distribution function from electron diffraction (ePDF) is a remarkable technique capable of elucidating the atomic arrangement materials with high spatial resolution, especially those that do not present sharp reflections, such as amorphous and nanoparticles. Higher electron-matter interaction favors use ePDF for have weak Bragg higher amounts diffuse scattering. The analysis treatment rec...

Electrochemical machining is based on the anodic dissolution of most metals and generates high quality polished surfaces. However, ferrite-perlite 42CrMo4 steel reveals local optical changes at surface after electrochemical finishing, such as a widely variable finish from shiny (reflective) to rough (dark) surfaces even one processing step. The different areas (AISI 4140) are studied by electro...

Off-axis electron holography in the transmission electron microscope is used to characterize the magnetic microstructure of magnetotactic bacteria. The practical details of the technique are illustrated through the examination of single cells of strains MV-1 and MS-1, which contain crystals of magnetite (Fe3O4) that are ~50 nm in size and are arranged in chains. Electron holography allows the m...

Open AccessCCS ChemistryRESEARCH ARTICLE5 Aug 2022Nanoporous Vesicular Membranes of Amphiphilic Polymers Containing Trans/Cis Isomers Hui Chen, Xia Yu, Yujiao Fan, Xiangjun Xing, Sylvain Trépout and Min-Hui Li Chen Beijing Advanced Innovation Center for Soft Matter Science Engineering, University Chemical Technology, Chaoyang District, 100029 Chimie ParisTech, PSL Paris, CNRS, Institut de Reche...

Serial block face scanning electron microscopy (SBF-SEM) is a powerful method to analyze cells in 3D. Here, working at the resolution limit of the method, we describe a correlative light-SBF-SEM workflow to resolve microtubules of the mitotic spindle in human cells. We present four examples of uses for this workflow which are not practical by light microscopy and/or transmission electron micros...

We report the use of chemical vapor deposition (CVD) for the bulk production (grams per day) of long, thin, and highly crystalline graphene ribbons (<20-30 μm in length) exhibiting widths of 20-300 nm and small thicknesses (2-40 layers). These layers usually exhibit perfect ABAB... stacking as in graphite crystals. The structure of the ribbons has been carefully characterized by several techniq...

Axial compression was conducted on micro-pillars, in which polycrystalline Cu thin films were sandwiched between CrN and Si. Plastic flow of Cu was achieved, when the Cu films are inclined either at 90° or 45° with respect to the pillar axis. The texture of Cu films was altered by changing the template on which film growth occurred. The Cu microstructure was further altered by post-deposition a...

22 The development of a successful vaccine against HIV-1 likely requires 23 immunogens that elicit both broadly neutralizing antibodies against envelope 24 spikes and T cell responses that recognize multiple viral proteins. HIV-1 virus like 25 particles (VLP), because they display authentic envelope spikes on the particle 26 surface, may be developed into such immunogens. However, in one way or...

Chronic cerebral hypoperfusion, a sustained modest reduction in cerebral blood flow, is associated with damage tomyelinated axons and cognitive decline with ageing. Oligodendrocytes (the myelin producing cells) and their precursor cells(OPCs) may be vulnerable to the effects of hypoperfusion and in some forms of injury OPCs have the potential to respondand repair damage by incre...

Background: Neuronal antibodies that show immunoreactivity across a broad range of species are important toolsfor comparative neuroanatomy. Nonetheless, the current antibody repertoire for non-model invertebrates is limited.Currently, only antibodies against the neuropeptide RFamide and the monoamine transmitter serotonin areextensively used. These antibodies label respective neuron...