The control of gastric pepsin secretion continues to excite controversy. The diversity in the results obtained by different investigators may be due, at least in part, to the varying techniques employed to estimate pepsin activity. Thus, edestin (Polland and Bloomfield, 1929), gelatin (Gilman and Cowgill, 1930), egg albumen (Vineberg and Babkin, 1931), beef haemoglobin (Anson and Mirsky, 1932),...

The nematode Ascaris suum primarily infects pigs, but also causes disease in humans. As part of its survival mechanism in the intestinal tract of the host, the worm produces a number of protease inhibitors, including pepsin inhibitor-3 (PI3), a 17 kDa protein. Recombinant PI3 expressed in E. coli has previously been shown to be a competitive inhibitor of a subgroup of aspartic proteinases: peps...

background and objective: chlorella vulgaris is a multi-cellular edible algal species with abundant proteins. extraction of high value protein fractions for pharmaceutical and nutritional applications can significantly increase the commercial value of microalga biomasses. there is no known report on the anticancer peptides derived from the chlorella vulgaris abundant protein. materials and meth...

In the presence of iodine at pH 5.0-6.0 a solution of pepsin absorbs iodine and the specific proteolytic activity of the solution decreases. The activity is less than 1 per cent of the original activity when the number of iodine atoms per mol of pepsin is 35-40. If the pH is 4.5 or less, iodine reacts very slowly and there is a correspondingly slower loss in activity. Glycyl tyrosine reacts wit...

Tsai, Tung-Lung, Shyue-Yih Chang, Chin-Yin Ho, and Yu Ru Kou. Neural and hydroxyl radical mechanisms underlying laryngeal airway hyperreactivity induced by laryngeal acid-pepsin insult in anesthetized rats. J Appl Physiol 101: 328 –338, 2006; doi:10.1152/japplphysiol.00064.2006.—Laryngopharyngeal or gastroesophageal reflux is associated with laryngeal airway hyperreactivity (LAH), but neither t...

In the words of Shepard the great sensitivity of Mycobacterium leprae to 4-4'-diaminodiphenyl sulfone (DDS) was at first surprising (13) . The DDS sensitivity of some other mycobacteria was shown by Karlson ( 3) to be much higher. Since many new species of mycobacteria have been described since 1963, we thought it would be useful to tes t these and to determine the minimal inhibitory concentrat...

Mycobacterium marinum isolates cultivated from tissue containing granulomatous lesions in Florida pompano Trachinotus carolinus and from biofilm samples collected from their tank and water recirculating system had identical (L1 of 11 bands) repetitive-sequence-based polymerase chain reaction (rep-PCR) DNA fingerprints. A second M. marinum clone sharing 4 of 11 rep-PCR bands with the first clone...

Few articles on concentration of pepsin in the gastric juice of patients with disease of the stomach and duodenum are to be found in recent literature, and the results reported by different observers are not always in accord. We shall consider here only the concentration of pepsin in the gastric juice of persons suffering with the syndrome of peptic ulcer. Wilcox (5), in 1908, reported that the...

A zymogen-derived protein, pepsin, appears to be incapable of folding to the native state without the presence of the prosegment. To better understand the nature of the irreversible denaturation of pepsin, the present study reports on the characterization of the stability and low-resolution tertiary and secondary structures of native, alkaline unfolded and acid refolded porcine pepsin. Through ...

Introduction. Gastroesophageal reflux has been associated with chronic inflammatory diseases and may be a cause of airway remodelling. Aspiration of gastric fluids may cause damage to airway epithelial cells, not only because acidity is toxic to bronchial epithelial cells, but also since it contains digestive enzymes, such as pepsin. Aim. To study whether pepsin enhances cytotoxicity and inflam...