UNLABELLED ᅟ: Starch requires six enzymes for digestion to free glucose: two amylases (salivary and pancreatic) and four mucosal maltase activities; sucrase-isomaltase and maltase-glucoamylase. All are deficient in suckling rodents. OBJECTIVE The objective of this study is to test (13)C-starch digestion before weaning by measuring enrichment of blood (13)C-glucose in maltase-glucoamylase-null...

Helsinki, Finland Ltd, POB 350, SF-00101 The hydrolysis of soluble starch, raw starch and pullulan with recombinant glucoamylase P from Hormoconis resinae was competitively inhibited by /?cyclodextrin with apparent Ki values of 190 pM, 13 pM and 1.4 pM, respectively. Inhibition of dextran hydrolysis was partial: a maximum inhibition of 22% was achieved with a dextran concentration of 0 3 x K, a...

A gene (ssg) encoding a putative glucoamylase in a hyperthermophilic archaeon, Sulfolobus solfataricus, was cloned and expressed in Escherichia coli, and the properties of the recombinant protein were examined in relation to the glucose production process. The recombinant glucoamylase was extremely thermostable, with an optimal temperature at 90 degrees C. The enzyme was most active in the pH r...

 Gregor Mendel. Conducted hybridization experiments on garden peas for seven years (1856 – 1863) and proposed laws of inheritance.  Mendel conducted artificial pollination/cross pollination experiments using several true-breeding pea lines.  A true breeding line is one that, having undergone continuous self-pollination for several generations.  Mendel selected 14 true-breeding peas’ plant v...

for treatment of anti-nutritional factors(anfs), (?-n-oxalyl -l- ?, ? -diaminopropionic acid (?-odap) and condensed tannin (ct)) in grass pea, some such alkali treatments as naoh, nahco3, kmno4 and wood ash, besides water soaking and polyethylene glycol (peg) (6000 mw) were employed . metabolizable energy (me) of treated and of untreated grass pea was assessed using gas production technique, tw...

Recombinant beta-carbonic anhydrase from the garden pea, Pisum sativum, was purified to homogeneity and crystallized. Crystals belong to the orthorhombic space group C222, with unit-cell parameters a = 136.3, b = 142.5, c = 201.4 A, alpha = beta = gamma = 90 degrees. Crystals typically diffracted anisotropically, with a maximal resolution of 2.0 A in the strongest direction. The calculated Matt...

Acridone synthase has been purified from cell suspension cultures of Ruta graveolens using a combination of gel filtration and ion exchange chromatography. The purified enzyme has an apparent molecular weight of 69 kDa on gel filtration and a subunit structure on SDS-PAGE of 40 kDa. The apparent Km-values are 10.64 microM and 32.8 microM for N-methylanthraniloyl-CoA and malonyl-CoA, respectivel...

BACKGROUND Amylases are used in various industrial processes and a key requirement for the efficiency of these processes is the use of enzymes with high catalytic activity at ambient temperature. Unfortunately, most amylases isolated from bacteria and filamentous fungi have optimal activity above 45 °C and low pH. For example, the most commonly used industrial glucoamylases, a type of amylase t...