Background: Subunit vaccines are appropriate vaccine candidates for the prevention of some infections. In this study, three immunogenic proteins of Mycobacterium tuberculosis, including HspX, Ppe44, and EsxV as a new construction, were expressed alone and as a fusion protein to develop a new vaccine candidate against tuberculosis infection. Methods: To make the fusion protein, the three genes ...

Background: Cutaneous leishmaniasis (CL) is a serious public health problem in many tropical countries. The infection is caused by a protozoan parasite of Leishmania genus transmitted by Phlebotominae sandflies. In the present study, we constructed a eukaryotic expression vector to produce a fusion protein containing LmSTI1 from Leishmania major (L. major) and PpSP42 from Phlebotomus papatasi (...

Preganglionic vagal motoneurones supplying the heart of the dogfish have been located in the medulla by antidromic stimulation of the central cut end of the branchial cardiac branch of the vagus. They supplied axons with conduction velocities between 4.75 and 16.3 m s-1, which is similar to mammalian B fibres. Motoneurones were found in two locations: the rostromedial (N = 5) and lateral (N = 1...

Craniofacial development is intrinsically related to segmentation along the primary body axis (Hunt and Krumlauf, 1991; Hunt et al., 1991a; Thorogood, 1993). In mammalian embryos, segmentally arranged cranial structures such as the branchial arches and nerve ganglia are built upon this primary metamerism (Keynes and Stern, 1984; Lumsden and Keynes, 1989; Puelles and Rubenstein, 1993). Like most...

Background: Conventional anti-cancer treatments, such as surgery, chemotherapy, radiation therapy, etc., are linked to antimicrobial resistance (AMR) and have negative effects on healthy tissues. Objectives: The flow cytometry technique was used in this study to evaluate the effectiveness of a modified anti-cancer peptide in treating the AGS gastric cancer cell line. Methods: This study used ...

objective: genes involved in bone and tissue remodelling in the vertebrates include matrix metalloproteinase-9 (mmp-9), receptor activator of necrosis factor κ-β (rank), cathepsin-k (ctsk) and tartrate-resistant acid phosphatase (tracp). we examine whether these markers are expressed in cells of zebrafish embryos of 1-5 days post fertilization. we also examine adult scales, which are known to c...

The study of material collected during routine monitoring surveys dealing with oil extraction and aquaculture in waters off Myanmar (North Andaman Sea) and in the Gulf of Thailand, respectively, allowed us to analyse the taxonomy and diversity of the polychaete genus Terebellides (Annelida). Three species were found, namely Terebellides cf. woolawa, Terebellides hutchingsae spec. nov. (a new sp...

objective: the aim of this study was to construct a pcdna3.1+ vector containing fmdv type o/irn/1/2007-vp1 gene, protein expression in bhkt7 cells and evaluation of immune response in balb/c mice. materials and methods: fmdv type o/irn/1/2007 was isolated from a cattle in ray in 2007 and serotyped. the purified vp1 gene was sub-cloned into the ptz57r/t vector and pcdna3.1+ expression vector. t...

acute lymphoblastic leukemia(all) is due to early stage arrest of lymphoblast development. the translocation of philadelphia (ph) chromosome occurs as a result of the bcr-abl fusion gene, which constitutively produced activated tyrosine kinase. this gene fusion is an important indicator for prognosis in all and is associated with poor overall survival and remission duration. bcr-abl could inter...