Studies were conducted using 68 males from the seventh generation of a line of meat-type breeder chickens selected for increased duration of fertility of frozen-thawed semen and 69 males of the randomly selected control line. These chickens were used to determine the presence or absence of differences between the selected and control lines with respect to fertility and spermatozoal motility whe...

The history of artificial insemination (AI) begins with a story about some Arabs stealing stallion semen from a rival tribe and bringing it home for breeding in a sponge. When AI began its modern development in Russia during the early 20th century by Ivanov, the main object was not cattle but horses as documented by Milovanov. In the late 1940s, Christopher Polge, working with chicken and the e...

Background: Frozen-thawed rooster semen is not reliable for use in artificial insemination in commercial stocks. Low density lipoprotein (LDL) has been assessed for effectiveness as a cryoprotectant in the extender to improve the quality of frozen-thawed rooster semen. Although LDL has been evaluated in a few studies in other species for semen cryopreservation, to date, no study has been conduc...

OBJECTIVE To evaluate the effectiveness of liquid nitrogen (LN(2)) vapor (indirect contact method) for the storage of human semen. DESIGN Experimental study. SETTING University hospital-based fertility center. PATIENT(S) We evaluated 150 patients with normal sperm parameters. INTERVENTION(S) Human semen (N = 120) was mixed with Semen Freezing Medium, divided into two groups, frozen, and...

Artificial insemination (AI) is becoming more common in canine reproduction, permitting the use of shipped semen, assistance for geriatric or subfertile males, coverage of dominant females, and advanced reproductive technology such as intrauterine deposition of semen. AI may be performed with fresh, chilled, or frozen semen. All instruments should be clean and free of any chemical contamination...

The freezability of semen collected during the low (May-July) and the peak (September-November) breeding seasons from 18 Nili-Ravi buffalo bulls of young (3-4 years), adult (6-8 years) and old ( 1215 years) age groups was studied. After extension in lactose.:..fructose-egg-yolk-glycerol extender, semen samples were frozen and stored in liquid nitrogen. Post-thaw motili�y and liveability of sper...

The purpose of this study was to investigate the effects of semen collection into tubes containing extender supplemented with BSA on the cryosurvival of goat spermatozoa. Semen was collected from two goats into empty tubes or tubes containing 10 ml extender supplemented with 0, 0.1, 1, or 5% BSA, and the washed spermatozoa were frozen as pellets in egg yolk-trehalose extender with the addition ...

The present study aimed to compare the fertility of ewes intrauterinally inseminated with frozen-thawed semen using a soybean-based semen extender (AndroMed) with those of ewes intrauterinally inseminated with frozen-thawed semen using a Tris-based extender containing either egg yolk or BSA. Suffolk ewes (n=104) were treated with an intravaginal sponge containing 40 mg fluoroprogesterone acetat...

The deleterious effects of three cryoprotectants, glycerol, dimethylsulfoxide (DMSO), and dimethylacetamide (DMA), were compared on fowl spermatozoa. The viability and integrity of spermatozoa were measured with eosin-nigrosin smears. Glycerol was the least deleterious cryoprotectant, followed by DMA, and DMSO was the most toxic. Methods employing either glycerol or DMA were then compared for t...

The effect of seminal plasma, as well as some seminal plasma fractions, on the heterogeneity and viability of frozen-thawed ram spermatozoa was studied. Fresh and frozen sperm samples were simultaneously assessed by a routine semen-quality assay and centrifugal countercurrent distribution (CCCD) in an aqueous two-phase system analysis. All samples used for this study were obtained from Salz ram...