The hypothesis was tested that frozen bovine oviduct epithelial cells (BOEC) could provide a physiologic and standardized system for studying motility and acrosomal changes of fresh and frozen-thawed sperm. The BOEC were collected by lavage from cows and monolayers prepared from both fresh and frozen-thawed cells. Fresh and frozen semen processed from five bulls was extended in a modified Tyrod...

A continuous decline in the number and range of capercaillie (Tetrao urogallus L.) in many European countries can be observed, mostly due to habitat destruction by human activity, unecological forestry management, and increased density of natural predators. Ex situ in vitro gene banks provide a unique opportunity to preserve the genetic material for future generations. Simple and effective cryo...

Bovine herpesvirus 1 (BoHV-1) and 5 (BoHV-5) are important pathogens of the respiratory and genital tract of cattle and may also affect the central nervous system and cause meningoencephalitis. Both virus types are estimated to be widely distributed in Southern Brazil. In the present study, BoHV-1 and/or BoHV-5 DNA were detected in bovine semen samples from two states of Brazil by two species-s...

Bovine Leukemia ProVirus: Evidence of Presence of Part of Gag Gene in Seminal Plasma of Naturally Infected Bulls It is of critical importance to understand the modalities of BLV presence in semen, especially with regard to artificial insemination (AI). Presence of bovine leukemia provirus was demonstrated in fresh and frozen semen samples by researchers. In this study paired blood and semen sam...

A fertility trial was conducted comparing two methods of defining the optimum time to inseminate frozen boar semen. One hundred sixty-four gilts were inseminated, one-half at a fixed insemination time of 32 to 34 h after calculated onset of estrus and the remainder were inseminated on the basis of changes in the vaginal mucosal electrical resistance. Inseminations were made when the instrument ...

Introduction Although dog was the first species to be used for Artificial Insemination (AI) and become pregnant by Spallanzani in 1780, very little work has been done on preservation of dog semen to exploit the full genetic potential of pedigree males. Frozen semen is now inseminated routinely in cattle, sheep and goats and much research has been carried out with regard to the optimum condition...

It is of critical importance to understand the modalities of BLV presence in semen, especially with regard to artificial insemination (AI). Presence of bovine leukemia provirus was demonstrated in fresh and frozen semen samples by researchers. In this study paired blood and semen samples from 45 bulls were assessed for the presence of part of gag gene and antibodies to BLV in blood, semen and c...

Somatic cells were isolated from cryopreserved semen of 4 buffalo bulls, 3 of which had died over 10 years earlier, and were established in culture. The cells expressed cytokeratin-18, keratin and vimentin indicating that they were of epithelial origin. The cells were used as nuclear donors for hand-made cloning for producing buffalo embryos. The blastocyst rate and quality, as indicated by apo...

The current study was performed to test the of quality as well as the DNA integrity of preserved liquid and frozen buffalo spermatozoa due to addition of different concentrations of glutathione (0.50, 1.00, 2.00 and 3.00 mM). Liquid semen was preserved for 120 hour with or without addition of glutathione and examined for progressive motility, viability, plasma and acrosomal membranes integrity ...

The effect of different phases of a freezing protocol on the fertilising ability of rabbit spermatozoa was evaluated. An extender containing 1.75 M DMSO and 0.05 M sucrose (final concentration) was used to freeze rabbit sperm. In the first experiment, the results obtained with fresh and cooled (5 degrees C for 45 min) sperm were compared; no differences were observed between fresh and cooled se...