In this paper, we evaluate the strategy of using self-assembled microbeads to build a robust and tunable membrane for free-flow zone electrophoresis in a PDMS microfluidic chip. To fabricate a porous membrane as a salt bridge for free-flow zone electrophoresis, we used silica or polystyrene microbeads between 3-6 μm in diameter and packed them inside a microchannel. After complete evaporation, ...

In mixing ventilation systems, diffusers are often located on side walls and supply quasi-free air jets above the occupied zone. The data presented in this paper shows a new CFD 3D benchmark with two well-defined characteristic zones in the room, i.e., the quasi-free jet zone and the occupied zone. Measurement methods adequate for air velocity and speed measurement were applied: laser Doppler a...

This study examined the physical and decision-making requirements of elite Australian football (AF) umpires during match play. Twenty-nine field umpires were assessed across 20 AF League matches. Physical demands were monitored using global positioning system devices to record the total distance covered and high-speed running (HSR; >14.4 km·h) demands across each quarter. Decision-making perfor...

Three major issues associated with numerical simulations of complex free-surface flows, viz. interface tracking, fragmentation and large physical jumps, are addressed by a new Hybrid continuum-particle model (HyPAM). The new model consists of three parts: (1) the Polygonal Area Mapping method [Q. Zhang and P. L.-F. Liu, A new interface tracking method: The polygonal area mapping method. J. Comp...

Introduction Exposure to chronic levels of ionizing radiation could be detrimental to health even at very low doses. Calabar free trade zone (CFTZ) was established to promote export business in Nigeria and it is yet to produce exposure data of the Zone. Materials and Methods The Zone was divided into three categories depending on the type of business. Category A had facilities with manufacturin...

In this paper, we are evaluating the strategy of sorting peptides/proteins based on the charge to mass without resorting to ampholytes and/or isoelectric focusing, using a single- and two-step free-flow zone electrophoresis. We developed a simple fabrication method to create a salt bridge for free-flow zone electrophoresis in PDMS chips by surface printing a hydrophobic layer on a glass substra...