Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Recovery after Photobleaching (FRAP) are probably the most widely used methods to determine diffusion coefficients in biological samples. However, it has already been noted in the past that the two methods do not necessarily provide the same results. This has typically been explained by the different spatial range over which the two m...

The gold standard to assess jaundice in neonates is the serum bilirubin measurement. Blood sampling for the determination of total serum bilirubin (TSB) is painful for newborns and stressful for parents. The Bilicheck®, a new transcutaneous bilirubinometer, is considered as a more accurate measurement of bilirubin compared to the previous bilirubinometers courtesy of its advanced technology. Th...

Seasonal discrepancy in the antioxidant potential from bark of Nothapodytes nimmoniana was evaluated using two different methods (DPPH and FRAP) and were related to total phenolic content as well as flavonoid content. Higher yield of phenolic and flavonoid were observed during summer (May) similarly, the antioxidant activities (DPPH & FRAP) also were high. The antioxidant activities were strong...

Fluorescence redistribution after photobleaching (FRAP) has received increasing attention ever since it was first introduced into cell biological research. The method was developed in the 1970s, when its biological application mainly focused on the mobility of fluorescently labelled constituents of the cell membrane. The development of confocal scanning microscopy in the 1980s facilitated accur...

hyperbilirubinemia is a common problem during neonatal period especially in preterm neonates. transcutaneous bilirubin measurement (tcb) by special devices had been documented as an effective tool for predicting neonatal jaundice in full term neonate, but for preterm infants the present evidences are controversial. we carried out a prospective study in shariati hospital nicu. 126 paired tcb/tot...

background: hyperbilirubinemia is a common physiological problem in approximately 80% of preterm infants during the first week after birth. increase in bowel movements reduces enterohepatic circulation and increases bilirubin excretion. aim: this study aimed to evaluate the effects of field massage technique on bilirubin level and the number of defecations in preterm infants method: this clinic...

Background: Neonatal hyperbilirubinemia is one of the most common causes of neonatal morbidity and a global health priority. This study aimed to evaluate the effect of intensive phototherapy on management of hyperbilirubinemia with different etiologies. Methods: This retrospective cohort study was conducted on 219 neonates with indirect hyperbilirubinemia, who had the indication for photothera...

Confocal or multi-photon laser scanning microscopes are convenient tools to perform FRAP diffusion measurements. Despite its popularity, accurate FRAP remains often challenging since current methods are either limited to relatively large bleach regions or can be complicated for non-specialists. In order to bring reliable quantitative FRAP measurements to the broad community of laser scanning mi...